Electrokinetic bioprocessor for concentrating cells and molecules

Pak Kin Wong, Che Yang Chen, Tza Huei Wang, Chih Ming Ho

Research output: Contribution to journalArticle

155 Scopus citations

Abstract

Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor then utilizes electrophoretic and dielectrophoretic forces, which are effective in short range, to trap the target cells and molecules on the electrode surface. By optimizing the operating parameters, we have concentrated various biological objects in a large range of sizes, including Escherichia coli bacteria, λ phage DNA, and single-stranded DNA fragments as small as 20 bases that have a radius of gyration of only 3 nm.

Original languageEnglish (US)
Pages (from-to)6908-6914
Number of pages7
JournalAnalytical chemistry
Volume76
Issue number23
DOIs
StatePublished - Jan 3 2005

ASJC Scopus subject areas

  • Analytical Chemistry

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