Electrophoretic analysis of ruminal degradability of corn proteins.

Donato Romagnolo, C. E. Polan, W. E. Barbeau

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Albumin, globulin, and prolamin fractions were extracted from corn meal in water, .5 M NaCl, and 50% (vol/vol) 1-propanol and examined by SDS-PAGE and densitometric scanning to investigate fractional degradation rates of corn proteins. Several protein fractions were identified for globulins, zein prolamins, and glutelins. Ruminal degradability of individual subfractions was evaluated by suspension of corn and corn gluten meal samples in the rumen of lactating dairy cows from 0 to 72 h. Electrophoretic and densitometric analysis of protein residues revealed that the prolamin fraction zein for corn and corn gluten meal was more resistant to ruminal degradation than albumins, globulins, and glutelins. Relative rates of degradation of zein and the fraction containing albumins, globulins, and glutelins were .060, .026, and .018, .015/h for corn and corn gluten meal, respectively. Total degradabilities of corn and corn gluten meal, measured by summation of degradability of subfractional components, were 52.2 and 18.6%. Quantitative measurement of ruminally degradable subfractions and estimation of their degradation rates by electrophoretic and densitometric scanning are useful in understanding ruminal degradability of corn proteins.

Original languageEnglish (US)
Pages (from-to)1093-1099
Number of pages7
JournalJournal of Dairy Science
Volume77
Issue number4
StatePublished - Apr 1994
Externally publishedYes

Fingerprint

corn protein
corn gluten meal
globulins
zein
glutelins
Zea mays
prolamins
Glutens
albumins
corn
degradation
Prolamins
Proteins
Globulins
Meals
Zein
1-propanol
corn meal
Albumins
rumen

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • veterinary(all)

Cite this

Electrophoretic analysis of ruminal degradability of corn proteins. / Romagnolo, Donato; Polan, C. E.; Barbeau, W. E.

In: Journal of Dairy Science, Vol. 77, No. 4, 04.1994, p. 1093-1099.

Research output: Contribution to journalArticle

Romagnolo, Donato ; Polan, C. E. ; Barbeau, W. E. / Electrophoretic analysis of ruminal degradability of corn proteins. In: Journal of Dairy Science. 1994 ; Vol. 77, No. 4. pp. 1093-1099.
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