Electrophysiological properties of human hypothalamic hamartomas

Jie Wu, Lin Xu, Do Young Kim, Jong M. Rho, Paul A St John, Lih Fen Lue, Stephen Coons, Kevin Ellsworth, Lee Nowak, Eric Johnson, Harold Rekate, John F. Kerrigan

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The hypothalamic hamartoma (HH) is a rare developmental malformation often characterized by gelastic seizures, which are usually refractory to medical therapy. The mechanisms of epileptogenesis operative in this subcortical lesion are unknown. In this study, we used standard patch-clamp electrophysiological techniques combined with histochemical approaches to study individual cells from human HH tissue immediately after surgical resection. More than 90% of dissociated HH cells were small (6-9μm soma) and exhibited immunoreactivity to the neuronal marker NeuN, and to glutamic acid decarboxylase, but not to glial fibrillary acidic protein. Under current-clamp, whole-cell recordings in single dissociated cells or in intact HH slices demonstrated typical neuronal responses to depolarizing and hyperpolarizing current injection. In some cases, HH cells exhibited a "sag-like" membrane potential change during membrane liyperpolarization. Interestingly, most HH cells exhibited robust, spontaneous "pacemaker-like" action potential firing. Under voltage-clamp, dissociated HH cells exhibited functional tetrodotoxin (TTX)-sensitive Na+ and tetraethylammonium-sensitive K+ currents. Both GABA and glutamate evoked whole-cell currents, with GABA exhibiting a peak current amplitude 10-fold greater than glutamate. These findings suggest that human HH tissues, associated with gelastic seizures, contained predominantly small GABAergic inhibitory neurons that exhibited intrinsic "pacemaker-like" behavior.

Original languageEnglish (US)
Pages (from-to)371-382
Number of pages12
JournalAnnals of Neurology
Volume58
Issue number3
DOIs
StatePublished - Sep 2005

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Laughter
Patch-Clamp Techniques
gamma-Aminobutyric Acid
Glutamic Acid
Seizures
GABAergic Neurons
Tetraethylammonium
Glutamate Decarboxylase
Hypothalamic hamartomas
Glial Fibrillary Acidic Protein
Tetrodotoxin
Carisoprodol
Membrane Potentials
Action Potentials
Injections
Membranes
Therapeutics

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wu, J., Xu, L., Kim, D. Y., Rho, J. M., St John, P. A., Lue, L. F., ... Kerrigan, J. F. (2005). Electrophysiological properties of human hypothalamic hamartomas. Annals of Neurology, 58(3), 371-382. https://doi.org/10.1002/ana.20580

Electrophysiological properties of human hypothalamic hamartomas. / Wu, Jie; Xu, Lin; Kim, Do Young; Rho, Jong M.; St John, Paul A; Lue, Lih Fen; Coons, Stephen; Ellsworth, Kevin; Nowak, Lee; Johnson, Eric; Rekate, Harold; Kerrigan, John F.

In: Annals of Neurology, Vol. 58, No. 3, 09.2005, p. 371-382.

Research output: Contribution to journalArticle

Wu, J, Xu, L, Kim, DY, Rho, JM, St John, PA, Lue, LF, Coons, S, Ellsworth, K, Nowak, L, Johnson, E, Rekate, H & Kerrigan, JF 2005, 'Electrophysiological properties of human hypothalamic hamartomas', Annals of Neurology, vol. 58, no. 3, pp. 371-382. https://doi.org/10.1002/ana.20580
Wu, Jie ; Xu, Lin ; Kim, Do Young ; Rho, Jong M. ; St John, Paul A ; Lue, Lih Fen ; Coons, Stephen ; Ellsworth, Kevin ; Nowak, Lee ; Johnson, Eric ; Rekate, Harold ; Kerrigan, John F. / Electrophysiological properties of human hypothalamic hamartomas. In: Annals of Neurology. 2005 ; Vol. 58, No. 3. pp. 371-382.
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