Elevated Sod2 activity augments matrix metalloproteinase expression: Evidence for the involvement of endogenous hydrogen peroxide in regulating metastasis

Kristin K. Nelson, Aparna R Sertil, Jelriza Mansouri, Ana M. Rodriguez, Kirwin M. Providence, Joni L. Rutter, Kevin Pumiglia, James A. Bennett, J. Andres Melendez

Research output: Contribution to journalArticle

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Abstract

Purpose: Elevated manganese superoxide dismutase (Sod2) levels have been reported to be associated with an increased frequency of tumor invasion and metastasis in certain cancers, and the aim of this study is to examine the molecular mechanisms by which this occurs. Experimental Design: Sod2 and catalase overexpressing HT-1080 fibrosarcoma cell lines were used to evaluate the H2O2-dependent regulation of matrix metalloproteinase (MMP)-1 promoter activity, mitogen-activated protein (MAP) kinase signaling, DNA-binding activity, and MMP mRNA levels. The invasive and metastatic potential of Sod2 overexpressing cells was characterized using subrenal capsular implantation or tail vein injection of tumor cells into nude mice, respectively. Results: Our data reveal that Sod2 overexpression increases the DNA-binding activity of transcription factors critical for MMP expression but also enhances MMP-1 promoter activity via the Ras//MAP/extracellular signal-regulated kinase (MEK) signaling cascade. A single nucleotide polymorphism that creates an Ets site at position - 1607 bp confers Sod2-dependent MMP-1 promoter activity. Sod2 overexpression also increases the mRNA levels of MMPs-2, -3, -7, -10, -9, -11 and enhances the metastatic potential of fibrosarcoma cells when implanted in immunodeficient mice. The Sod2-dependent increases in AP-1 and SP-1 DNA-binding activity, MMP-1 promoter activity, general MMP expression, and collagen degradation can be reversed by the hydrogen peroxide-detoxifying enzyme, catalase. Conclusion: MMPs play a critical role in the process of stromal invasion and metastasis, and these findings suggest that the association between increased Sod2 and poor prognosis in certain cancers may be attributed to elevated MMP production.

Original languageEnglish (US)
Pages (from-to)424-432
Number of pages9
JournalClinical Cancer Research
Volume9
Issue number1 I
StatePublished - Jan 1 2003
Externally publishedYes

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Matrix Metalloproteinases
Hydrogen Peroxide
Matrix Metalloproteinase 1
Neoplasm Metastasis
Fibrosarcoma
Catalase
DNA
Neoplasms
Messenger RNA
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Transcription Factor AP-1
Mitogen-Activated Protein Kinases
Mitogens
Nude Mice
Superoxide Dismutase
Single Nucleotide Polymorphism
Tail
Veins
Research Design

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nelson, K. K., Sertil, A. R., Mansouri, J., Rodriguez, A. M., Providence, K. M., Rutter, J. L., ... Melendez, J. A. (2003). Elevated Sod2 activity augments matrix metalloproteinase expression: Evidence for the involvement of endogenous hydrogen peroxide in regulating metastasis. Clinical Cancer Research, 9(1 I), 424-432.

Elevated Sod2 activity augments matrix metalloproteinase expression : Evidence for the involvement of endogenous hydrogen peroxide in regulating metastasis. / Nelson, Kristin K.; Sertil, Aparna R; Mansouri, Jelriza; Rodriguez, Ana M.; Providence, Kirwin M.; Rutter, Joni L.; Pumiglia, Kevin; Bennett, James A.; Melendez, J. Andres.

In: Clinical Cancer Research, Vol. 9, No. 1 I, 01.01.2003, p. 424-432.

Research output: Contribution to journalArticle

Nelson, KK, Sertil, AR, Mansouri, J, Rodriguez, AM, Providence, KM, Rutter, JL, Pumiglia, K, Bennett, JA & Melendez, JA 2003, 'Elevated Sod2 activity augments matrix metalloproteinase expression: Evidence for the involvement of endogenous hydrogen peroxide in regulating metastasis', Clinical Cancer Research, vol. 9, no. 1 I, pp. 424-432.
Nelson, Kristin K. ; Sertil, Aparna R ; Mansouri, Jelriza ; Rodriguez, Ana M. ; Providence, Kirwin M. ; Rutter, Joni L. ; Pumiglia, Kevin ; Bennett, James A. ; Melendez, J. Andres. / Elevated Sod2 activity augments matrix metalloproteinase expression : Evidence for the involvement of endogenous hydrogen peroxide in regulating metastasis. In: Clinical Cancer Research. 2003 ; Vol. 9, No. 1 I. pp. 424-432.
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abstract = "Purpose: Elevated manganese superoxide dismutase (Sod2) levels have been reported to be associated with an increased frequency of tumor invasion and metastasis in certain cancers, and the aim of this study is to examine the molecular mechanisms by which this occurs. Experimental Design: Sod2 and catalase overexpressing HT-1080 fibrosarcoma cell lines were used to evaluate the H2O2-dependent regulation of matrix metalloproteinase (MMP)-1 promoter activity, mitogen-activated protein (MAP) kinase signaling, DNA-binding activity, and MMP mRNA levels. The invasive and metastatic potential of Sod2 overexpressing cells was characterized using subrenal capsular implantation or tail vein injection of tumor cells into nude mice, respectively. Results: Our data reveal that Sod2 overexpression increases the DNA-binding activity of transcription factors critical for MMP expression but also enhances MMP-1 promoter activity via the Ras//MAP/extracellular signal-regulated kinase (MEK) signaling cascade. A single nucleotide polymorphism that creates an Ets site at position - 1607 bp confers Sod2-dependent MMP-1 promoter activity. Sod2 overexpression also increases the mRNA levels of MMPs-2, -3, -7, -10, -9, -11 and enhances the metastatic potential of fibrosarcoma cells when implanted in immunodeficient mice. The Sod2-dependent increases in AP-1 and SP-1 DNA-binding activity, MMP-1 promoter activity, general MMP expression, and collagen degradation can be reversed by the hydrogen peroxide-detoxifying enzyme, catalase. Conclusion: MMPs play a critical role in the process of stromal invasion and metastasis, and these findings suggest that the association between increased Sod2 and poor prognosis in certain cancers may be attributed to elevated MMP production.",
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AU - Sertil, Aparna R

AU - Mansouri, Jelriza

AU - Rodriguez, Ana M.

AU - Providence, Kirwin M.

AU - Rutter, Joni L.

AU - Pumiglia, Kevin

AU - Bennett, James A.

AU - Melendez, J. Andres

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N2 - Purpose: Elevated manganese superoxide dismutase (Sod2) levels have been reported to be associated with an increased frequency of tumor invasion and metastasis in certain cancers, and the aim of this study is to examine the molecular mechanisms by which this occurs. Experimental Design: Sod2 and catalase overexpressing HT-1080 fibrosarcoma cell lines were used to evaluate the H2O2-dependent regulation of matrix metalloproteinase (MMP)-1 promoter activity, mitogen-activated protein (MAP) kinase signaling, DNA-binding activity, and MMP mRNA levels. The invasive and metastatic potential of Sod2 overexpressing cells was characterized using subrenal capsular implantation or tail vein injection of tumor cells into nude mice, respectively. Results: Our data reveal that Sod2 overexpression increases the DNA-binding activity of transcription factors critical for MMP expression but also enhances MMP-1 promoter activity via the Ras//MAP/extracellular signal-regulated kinase (MEK) signaling cascade. A single nucleotide polymorphism that creates an Ets site at position - 1607 bp confers Sod2-dependent MMP-1 promoter activity. Sod2 overexpression also increases the mRNA levels of MMPs-2, -3, -7, -10, -9, -11 and enhances the metastatic potential of fibrosarcoma cells when implanted in immunodeficient mice. The Sod2-dependent increases in AP-1 and SP-1 DNA-binding activity, MMP-1 promoter activity, general MMP expression, and collagen degradation can be reversed by the hydrogen peroxide-detoxifying enzyme, catalase. Conclusion: MMPs play a critical role in the process of stromal invasion and metastasis, and these findings suggest that the association between increased Sod2 and poor prognosis in certain cancers may be attributed to elevated MMP production.

AB - Purpose: Elevated manganese superoxide dismutase (Sod2) levels have been reported to be associated with an increased frequency of tumor invasion and metastasis in certain cancers, and the aim of this study is to examine the molecular mechanisms by which this occurs. Experimental Design: Sod2 and catalase overexpressing HT-1080 fibrosarcoma cell lines were used to evaluate the H2O2-dependent regulation of matrix metalloproteinase (MMP)-1 promoter activity, mitogen-activated protein (MAP) kinase signaling, DNA-binding activity, and MMP mRNA levels. The invasive and metastatic potential of Sod2 overexpressing cells was characterized using subrenal capsular implantation or tail vein injection of tumor cells into nude mice, respectively. Results: Our data reveal that Sod2 overexpression increases the DNA-binding activity of transcription factors critical for MMP expression but also enhances MMP-1 promoter activity via the Ras//MAP/extracellular signal-regulated kinase (MEK) signaling cascade. A single nucleotide polymorphism that creates an Ets site at position - 1607 bp confers Sod2-dependent MMP-1 promoter activity. Sod2 overexpression also increases the mRNA levels of MMPs-2, -3, -7, -10, -9, -11 and enhances the metastatic potential of fibrosarcoma cells when implanted in immunodeficient mice. The Sod2-dependent increases in AP-1 and SP-1 DNA-binding activity, MMP-1 promoter activity, general MMP expression, and collagen degradation can be reversed by the hydrogen peroxide-detoxifying enzyme, catalase. Conclusion: MMPs play a critical role in the process of stromal invasion and metastasis, and these findings suggest that the association between increased Sod2 and poor prognosis in certain cancers may be attributed to elevated MMP production.

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