Elicitation of anti-H-2 cytotoxic T lymphocytes with antigen-modified H-2 negative stimulator cells

A. H. Hale, M. J. Ruebush, David T. Harris, M. P. McGee

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4 Scopus citations


By a newly developed technique, we have modified H-2(k)-negative cells in such a way as to render them capable of elicitation of anti-H-2 CTL. Purified H-2K(k) and/or partially purified Ia(k) glycoproteins were incorporated into lipid vesicles that contained the hemagglutinin-neuraminidase (HN) glycoprotein and the fusion (F) glycoprotein of Sendai virus. These lipid vesicles were incubated with H-2-negative tumor cells (R1-) in a manner that resulted in rendering these cells capable of eliciting anti-H-2K(k) CTL. The pressure of Ia(k) on the surface of the modified stimulator cell had a significant effect on the proportion of anti-H-2K(k) CTL possessing a particular Lyt phenotype. Primary effector cells elicited by an H-2K(k)-Ia(k) difference were primarily Lyt 1-, 2+, whereas primary anti-H-2K(k) CTL elicited by an H-2K(k) difference alone were predominantly Lyt 1+, 2+.

Original languageEnglish (US)
Pages (from-to)1485-1488
Number of pages4
JournalJournal of Immunology
Issue number4
Publication statusPublished - 1981
Externally publishedYes


ASJC Scopus subject areas

  • Immunology

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