EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7

Diane E. Shevell, Wei Ming Leu, C. Stewart Gillmor, Guixian Xia, Kenneth A Feldmann, Nam Hai Chua

Research output: Contribution to journalArticle

245 Citations (Scopus)

Abstract

The EMB30 gene is involved in apical-basal pattern formation in the Arabidopsis embryo. Mutations in this locus produce mutants with a wide range of seedling phenotypes, but all of the mutants lack a root and a true hypocotyl. We have cloned the EMB30 gene, and it encodes a protein that has similarity to the yeast Sec7 protein and to two other open reading frames identified in clones from humans and C. elegans. We refer to the region of similarity among these four sequences as the Sec7 domain. The emb30-1 allele has a mutation in the Sec7 domain that alters a residue conserved in all four of these sequences, suggesting that this domain may be important for EMB30 function. Molecular data and microscopy studies of emb30 seedlings presented here indicate that EMB30 affects cell division, elongation, and adhesion and functions in seedling and adult plants as well as during embryogenic pattern formation.

Original languageEnglish (US)
Pages (from-to)1051-1062
Number of pages12
JournalCell
Volume77
Issue number7
DOIs
StatePublished - Jul 1 1994

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Cell adhesion
Seedlings
Arabidopsis
Cell Adhesion
Cell Division
Genes
Cells
Hypocotyl
Elongation
Microscopic examination
Mutation
Proteins
Fungal Proteins
Adhesion
Open Reading Frames
Microscopy
Embryonic Structures
Clone Cells
Alleles
Phenotype

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7. / Shevell, Diane E.; Leu, Wei Ming; Gillmor, C. Stewart; Xia, Guixian; Feldmann, Kenneth A; Chua, Nam Hai.

In: Cell, Vol. 77, No. 7, 01.07.1994, p. 1051-1062.

Research output: Contribution to journalArticle

Shevell, Diane E. ; Leu, Wei Ming ; Gillmor, C. Stewart ; Xia, Guixian ; Feldmann, Kenneth A ; Chua, Nam Hai. / EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7. In: Cell. 1994 ; Vol. 77, No. 7. pp. 1051-1062.
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abstract = "The EMB30 gene is involved in apical-basal pattern formation in the Arabidopsis embryo. Mutations in this locus produce mutants with a wide range of seedling phenotypes, but all of the mutants lack a root and a true hypocotyl. We have cloned the EMB30 gene, and it encodes a protein that has similarity to the yeast Sec7 protein and to two other open reading frames identified in clones from humans and C. elegans. We refer to the region of similarity among these four sequences as the Sec7 domain. The emb30-1 allele has a mutation in the Sec7 domain that alters a residue conserved in all four of these sequences, suggesting that this domain may be important for EMB30 function. Molecular data and microscopy studies of emb30 seedlings presented here indicate that EMB30 affects cell division, elongation, and adhesion and functions in seedling and adult plants as well as during embryogenic pattern formation.",
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AU - Feldmann, Kenneth A

AU - Chua, Nam Hai

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