Embryonic expression of the chicken Krüppel-like (KLF) transcription factor gene family

Parker B. Antin, Maricela Pier, Terry Sesepasara, Tatiana A. Yatskievych, Diana K. Darnell

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The Krüppel-like transcription factors (KLF) are zinc finger proteins that activate and suppress target gene transcription. Although KLF factors have been implicated in regulating many developmental processes, a comprehensive gene expression analysis has not been reported. Here we present the chicken KLF gene family and expression during the first five days of embryonic development. Fourteen chicken KLF genes or expressed sequences have been previously identified. Through synteny analysis and cDNA mapping, we have identified the KLF9 gene and determined that the gene presently named KLF1 is the true ortholog of KLF17 in other species. In situ hybridization expression analyses show that in general KLFs are broadly expressed in multiple cell and tissue types. Expression of KLFs 3, 7, 8, and 9, is widespread at all stages examined. KLFs 2, 4, 5, 6, 10, 11, 15, and 17 show more restricted patterns that suggest multiple functions during early stages of embryonic development.

Original languageEnglish (US)
Pages (from-to)1879-1887
Number of pages9
JournalDevelopmental Dynamics
Volume239
Issue number6
DOIs
StatePublished - Jun 2010

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Keywords

  • Chicken embryo
  • In situ hybridization
  • KLF
  • Krüppel-like factor

ASJC Scopus subject areas

  • Developmental Biology

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