Embryonic origins of spleen asymmetry

Kristin D. Patterson, Thomas A. Drysdale, Paul A Krieg

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The spleen is a vertebrate organ that has both hematopoietic and immunologic function. The embryonic origins of the spleen are obscure, with most studies describing the earliest rudiment of the spleen as a condensation of mesodermal mesenchyme on the left side of the dorsal mesogastrium. The development of spleen handedness has not been described previously, presumably because of the difficulty in assaying spleen position in the embryo and the lack of early, organ-specific molecular markers. Here we show that expression of the homeobox gene Nkx2-5 serves as a marker for spleen precursor tissue. Pre-splenic tissue is initially located in symmetric domains on both sides of the embryo but, during subsequent development, only the left side goes on to form the mature spleen. Therefore, the final location of the spleen on the left side of the body axis appears to result from preferential development of the spleen precursor cells on the left side of the embryo. Our studies indicate that the spleen and heart become asymmetric via different cellular mechanisms, Nkx2-5 may function locally as part of the laterality cascade, downstream of nodal and Pitx2, or it may direct asymmetric morphogenesis after laterality has been determined.

Original languageEnglish (US)
Pages (from-to)167-175
Number of pages9
JournalDevelopment
Volume127
Issue number1
StatePublished - Jan 2000

Fingerprint

Spleen
Embryonic Structures
Functional Laterality
Homeobox Genes
Mesoderm
Morphogenesis
Vertebrates

Keywords

  • Homeobox gene
  • Left/right axis
  • Nkx2-5
  • Polysplenia
  • Spleen
  • tinman
  • Vertebrate

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Patterson, K. D., Drysdale, T. A., & Krieg, P. A. (2000). Embryonic origins of spleen asymmetry. Development, 127(1), 167-175.

Embryonic origins of spleen asymmetry. / Patterson, Kristin D.; Drysdale, Thomas A.; Krieg, Paul A.

In: Development, Vol. 127, No. 1, 01.2000, p. 167-175.

Research output: Contribution to journalArticle

Patterson, KD, Drysdale, TA & Krieg, PA 2000, 'Embryonic origins of spleen asymmetry', Development, vol. 127, no. 1, pp. 167-175.
Patterson KD, Drysdale TA, Krieg PA. Embryonic origins of spleen asymmetry. Development. 2000 Jan;127(1):167-175.
Patterson, Kristin D. ; Drysdale, Thomas A. ; Krieg, Paul A. / Embryonic origins of spleen asymmetry. In: Development. 2000 ; Vol. 127, No. 1. pp. 167-175.
@article{f8680272abf94c5d80e8dec58cf7c2b1,
title = "Embryonic origins of spleen asymmetry",
abstract = "The spleen is a vertebrate organ that has both hematopoietic and immunologic function. The embryonic origins of the spleen are obscure, with most studies describing the earliest rudiment of the spleen as a condensation of mesodermal mesenchyme on the left side of the dorsal mesogastrium. The development of spleen handedness has not been described previously, presumably because of the difficulty in assaying spleen position in the embryo and the lack of early, organ-specific molecular markers. Here we show that expression of the homeobox gene Nkx2-5 serves as a marker for spleen precursor tissue. Pre-splenic tissue is initially located in symmetric domains on both sides of the embryo but, during subsequent development, only the left side goes on to form the mature spleen. Therefore, the final location of the spleen on the left side of the body axis appears to result from preferential development of the spleen precursor cells on the left side of the embryo. Our studies indicate that the spleen and heart become asymmetric via different cellular mechanisms, Nkx2-5 may function locally as part of the laterality cascade, downstream of nodal and Pitx2, or it may direct asymmetric morphogenesis after laterality has been determined.",
keywords = "Homeobox gene, Left/right axis, Nkx2-5, Polysplenia, Spleen, tinman, Vertebrate",
author = "Patterson, {Kristin D.} and Drysdale, {Thomas A.} and Krieg, {Paul A}",
year = "2000",
month = "1",
language = "English (US)",
volume = "127",
pages = "167--175",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "1",

}

TY - JOUR

T1 - Embryonic origins of spleen asymmetry

AU - Patterson, Kristin D.

AU - Drysdale, Thomas A.

AU - Krieg, Paul A

PY - 2000/1

Y1 - 2000/1

N2 - The spleen is a vertebrate organ that has both hematopoietic and immunologic function. The embryonic origins of the spleen are obscure, with most studies describing the earliest rudiment of the spleen as a condensation of mesodermal mesenchyme on the left side of the dorsal mesogastrium. The development of spleen handedness has not been described previously, presumably because of the difficulty in assaying spleen position in the embryo and the lack of early, organ-specific molecular markers. Here we show that expression of the homeobox gene Nkx2-5 serves as a marker for spleen precursor tissue. Pre-splenic tissue is initially located in symmetric domains on both sides of the embryo but, during subsequent development, only the left side goes on to form the mature spleen. Therefore, the final location of the spleen on the left side of the body axis appears to result from preferential development of the spleen precursor cells on the left side of the embryo. Our studies indicate that the spleen and heart become asymmetric via different cellular mechanisms, Nkx2-5 may function locally as part of the laterality cascade, downstream of nodal and Pitx2, or it may direct asymmetric morphogenesis after laterality has been determined.

AB - The spleen is a vertebrate organ that has both hematopoietic and immunologic function. The embryonic origins of the spleen are obscure, with most studies describing the earliest rudiment of the spleen as a condensation of mesodermal mesenchyme on the left side of the dorsal mesogastrium. The development of spleen handedness has not been described previously, presumably because of the difficulty in assaying spleen position in the embryo and the lack of early, organ-specific molecular markers. Here we show that expression of the homeobox gene Nkx2-5 serves as a marker for spleen precursor tissue. Pre-splenic tissue is initially located in symmetric domains on both sides of the embryo but, during subsequent development, only the left side goes on to form the mature spleen. Therefore, the final location of the spleen on the left side of the body axis appears to result from preferential development of the spleen precursor cells on the left side of the embryo. Our studies indicate that the spleen and heart become asymmetric via different cellular mechanisms, Nkx2-5 may function locally as part of the laterality cascade, downstream of nodal and Pitx2, or it may direct asymmetric morphogenesis after laterality has been determined.

KW - Homeobox gene

KW - Left/right axis

KW - Nkx2-5

KW - Polysplenia

KW - Spleen

KW - tinman

KW - Vertebrate

UR - http://www.scopus.com/inward/record.url?scp=0033959285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033959285&partnerID=8YFLogxK

M3 - Article

VL - 127

SP - 167

EP - 175

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 1

ER -