Embryonic stem cells as a model for cardiogenesis

Jeffrey Robbins, Thomas C Doetschman, W. Keith Jones, Alejandro Sánchez

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Embryonic stem (ES) cells are derived from the inner cell mass of mouse blastocysts. These cells, when placed upon a suitable fibroblast feeder layer, continue to proliferate without overt differentiation and remain totipotent. Cells in this state are competent for gene targeting via homologous recombination. Hence, they hold the possibility of developing defined animal models of human cardiovascular disease. When removed from the feeder layer, ES cells undergo differentiation and development into large, multicellular structures, termed embryoid bodies (EBs). Morphologic, biochemical, and molecular genetic analyses indicate that during EB development some early aspects of cardiogenesis are recapitulated. Thus, EB development in culture is useful for studying certain early cardiogenic events.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalTrends in Cardiovascular Medicine
Volume2
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Embryoid Bodies
Embryonic Stem Cells
Feeder Cells
Molecular Biology
Blastocyst Inner Cell Mass
Gene Targeting
Homologous Recombination
Cell Differentiation
Cardiovascular Diseases
Animal Models
Fibroblasts

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Embryonic stem cells as a model for cardiogenesis. / Robbins, Jeffrey; Doetschman, Thomas C; Jones, W. Keith; Sánchez, Alejandro.

In: Trends in Cardiovascular Medicine, Vol. 2, No. 2, 1992, p. 44-50.

Research output: Contribution to journalArticle

Robbins, Jeffrey ; Doetschman, Thomas C ; Jones, W. Keith ; Sánchez, Alejandro. / Embryonic stem cells as a model for cardiogenesis. In: Trends in Cardiovascular Medicine. 1992 ; Vol. 2, No. 2. pp. 44-50.
@article{af0fd7e1074a40eba84c8c15631f24e0,
title = "Embryonic stem cells as a model for cardiogenesis",
abstract = "Embryonic stem (ES) cells are derived from the inner cell mass of mouse blastocysts. These cells, when placed upon a suitable fibroblast feeder layer, continue to proliferate without overt differentiation and remain totipotent. Cells in this state are competent for gene targeting via homologous recombination. Hence, they hold the possibility of developing defined animal models of human cardiovascular disease. When removed from the feeder layer, ES cells undergo differentiation and development into large, multicellular structures, termed embryoid bodies (EBs). Morphologic, biochemical, and molecular genetic analyses indicate that during EB development some early aspects of cardiogenesis are recapitulated. Thus, EB development in culture is useful for studying certain early cardiogenic events.",
author = "Jeffrey Robbins and Doetschman, {Thomas C} and Jones, {W. Keith} and Alejandro S{\'a}nchez",
year = "1992",
doi = "10.1016/1050-1738(92)90003-B",
language = "English (US)",
volume = "2",
pages = "44--50",
journal = "Trends in Cardiovascular Medicine",
issn = "1050-1738",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Embryonic stem cells as a model for cardiogenesis

AU - Robbins, Jeffrey

AU - Doetschman, Thomas C

AU - Jones, W. Keith

AU - Sánchez, Alejandro

PY - 1992

Y1 - 1992

N2 - Embryonic stem (ES) cells are derived from the inner cell mass of mouse blastocysts. These cells, when placed upon a suitable fibroblast feeder layer, continue to proliferate without overt differentiation and remain totipotent. Cells in this state are competent for gene targeting via homologous recombination. Hence, they hold the possibility of developing defined animal models of human cardiovascular disease. When removed from the feeder layer, ES cells undergo differentiation and development into large, multicellular structures, termed embryoid bodies (EBs). Morphologic, biochemical, and molecular genetic analyses indicate that during EB development some early aspects of cardiogenesis are recapitulated. Thus, EB development in culture is useful for studying certain early cardiogenic events.

AB - Embryonic stem (ES) cells are derived from the inner cell mass of mouse blastocysts. These cells, when placed upon a suitable fibroblast feeder layer, continue to proliferate without overt differentiation and remain totipotent. Cells in this state are competent for gene targeting via homologous recombination. Hence, they hold the possibility of developing defined animal models of human cardiovascular disease. When removed from the feeder layer, ES cells undergo differentiation and development into large, multicellular structures, termed embryoid bodies (EBs). Morphologic, biochemical, and molecular genetic analyses indicate that during EB development some early aspects of cardiogenesis are recapitulated. Thus, EB development in culture is useful for studying certain early cardiogenic events.

UR - http://www.scopus.com/inward/record.url?scp=0026603780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026603780&partnerID=8YFLogxK

U2 - 10.1016/1050-1738(92)90003-B

DO - 10.1016/1050-1738(92)90003-B

M3 - Article

C2 - 21239273

AN - SCOPUS:0026603780

VL - 2

SP - 44

EP - 50

JO - Trends in Cardiovascular Medicine

JF - Trends in Cardiovascular Medicine

SN - 1050-1738

IS - 2

ER -