ENaC in the rabbit lacrimal gland and its changes during sjögren syndrome and pregnancy

Mingwu Wang, Jianyan Huang, Michael Lu, Shunhua Zhang, Chuanqing Ding

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Epithelial sodium channel (ENaC) plays a critical role in the control of Na + balance and the development and progression of exocrine gland pathologic condition. The aim of the present study was to investigate the presence of ENaC in the rabbit lacrimal gland (LG) and its potential changes during induced autoimmune dacryoadenitis (IAD) and pregnancy. Methods: Total messenger RNA (mRNA) of α, β, and γ subunits was extracted from whole LG, acinar cells, and ductal cells by laser capture microdissection (LCM) for real-time reverse-transcriptase polymerase chain reaction. Lacrimal glands were processed for Western blot and immunofluorescence. Results: Messenger RNA for both α and γ was expressed in whole LG lysates, whereas β was undetectable. In rabbits with IAD, the levels of mRNA for α and γ were 20.9% and 58.9% lower (P

Original languageEnglish (US)
Pages (from-to)297-303
Number of pages7
JournalEye and Contact Lens
Volume41
Issue number5
DOIs
StatePublished - Sep 11 2015

Fingerprint

Lacrimal Apparatus
Dacryocystitis
Rabbits
Pregnancy
Messenger RNA
Exocrine Glands
Laser Capture Microdissection
Epithelial Sodium Channels
Acinar Cells
Reverse Transcriptase Polymerase Chain Reaction
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Western Blotting

Keywords

  • Dry eye
  • Epithelial sodium channel
  • Lacrimal gland
  • Pregnancy
  • Sjögren syndrome

ASJC Scopus subject areas

  • Ophthalmology

Cite this

ENaC in the rabbit lacrimal gland and its changes during sjögren syndrome and pregnancy. / Wang, Mingwu; Huang, Jianyan; Lu, Michael; Zhang, Shunhua; Ding, Chuanqing.

In: Eye and Contact Lens, Vol. 41, No. 5, 11.09.2015, p. 297-303.

Research output: Contribution to journalArticle

Wang, Mingwu ; Huang, Jianyan ; Lu, Michael ; Zhang, Shunhua ; Ding, Chuanqing. / ENaC in the rabbit lacrimal gland and its changes during sjögren syndrome and pregnancy. In: Eye and Contact Lens. 2015 ; Vol. 41, No. 5. pp. 297-303.
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