Endogenous IAA levels and development of coffee flower buds from dormancy to anthesis

Ursula K Schuch, Anita N. Azarenko, Leslie H. Fuchigami

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Dormant coffee (Coffea arabica L.) flower buds require water stress to stimulate regrowth. A xylem specific water-soluble dye, azosulfamide, was used to quantify water uptake of buds after their release from dormancy by water stress. In non-stressed flower buds, the rate of water uptake was generally slower and variable compared to stressed flower buds, where the rate of uptake tripled from 1 to 3 days after rewatering and preceded the doubling of fresh and dry weight of buds. Free, ester and amide IAA levels of developing flower buds were measured by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution technique with [13C6]IAA as an internal standard. Throughout development, the majority of IAA was present as amide IAA. The proportions of amide and free IAA increased one day after plants were released from water stress, and preceded the doubling of fresh and dry weight. Free and conjugated IAA content per bud remained stable during the period of rapid flower growth until one day before anthesis.

Original languageEnglish (US)
Pages (from-to)33-41
Number of pages9
JournalPlant Growth Regulation
Volume15
Issue number1
DOIs
StatePublished - Jul 1994
Externally publishedYes

Fingerprint

Coffee
dormancy
buds
flowering
Dehydration
Amides
amides
Water
water stress
Coffea
water uptake
Weights and Measures
Xylem
Indicator Dilution Techniques
Isotopes
Gas Chromatography-Mass Spectrometry
isotope dilution technique
Esters
Coloring Agents
Coffea arabica

Keywords

  • Coffea arabica
  • flower bud dormancy
  • water stress

ASJC Scopus subject areas

  • Plant Science

Cite this

Endogenous IAA levels and development of coffee flower buds from dormancy to anthesis. / Schuch, Ursula K; Azarenko, Anita N.; Fuchigami, Leslie H.

In: Plant Growth Regulation, Vol. 15, No. 1, 07.1994, p. 33-41.

Research output: Contribution to journalArticle

Schuch, Ursula K ; Azarenko, Anita N. ; Fuchigami, Leslie H. / Endogenous IAA levels and development of coffee flower buds from dormancy to anthesis. In: Plant Growth Regulation. 1994 ; Vol. 15, No. 1. pp. 33-41.
@article{a148c6c50f644d5398c9195ca3edcfd9,
title = "Endogenous IAA levels and development of coffee flower buds from dormancy to anthesis",
abstract = "Dormant coffee (Coffea arabica L.) flower buds require water stress to stimulate regrowth. A xylem specific water-soluble dye, azosulfamide, was used to quantify water uptake of buds after their release from dormancy by water stress. In non-stressed flower buds, the rate of water uptake was generally slower and variable compared to stressed flower buds, where the rate of uptake tripled from 1 to 3 days after rewatering and preceded the doubling of fresh and dry weight of buds. Free, ester and amide IAA levels of developing flower buds were measured by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution technique with [13C6]IAA as an internal standard. Throughout development, the majority of IAA was present as amide IAA. The proportions of amide and free IAA increased one day after plants were released from water stress, and preceded the doubling of fresh and dry weight. Free and conjugated IAA content per bud remained stable during the period of rapid flower growth until one day before anthesis.",
keywords = "Coffea arabica, flower bud dormancy, water stress",
author = "Schuch, {Ursula K} and Azarenko, {Anita N.} and Fuchigami, {Leslie H.}",
year = "1994",
month = "7",
doi = "10.1007/BF00024674",
language = "English (US)",
volume = "15",
pages = "33--41",
journal = "Plant Growth Regulation",
issn = "0167-6903",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Endogenous IAA levels and development of coffee flower buds from dormancy to anthesis

AU - Schuch, Ursula K

AU - Azarenko, Anita N.

AU - Fuchigami, Leslie H.

PY - 1994/7

Y1 - 1994/7

N2 - Dormant coffee (Coffea arabica L.) flower buds require water stress to stimulate regrowth. A xylem specific water-soluble dye, azosulfamide, was used to quantify water uptake of buds after their release from dormancy by water stress. In non-stressed flower buds, the rate of water uptake was generally slower and variable compared to stressed flower buds, where the rate of uptake tripled from 1 to 3 days after rewatering and preceded the doubling of fresh and dry weight of buds. Free, ester and amide IAA levels of developing flower buds were measured by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution technique with [13C6]IAA as an internal standard. Throughout development, the majority of IAA was present as amide IAA. The proportions of amide and free IAA increased one day after plants were released from water stress, and preceded the doubling of fresh and dry weight. Free and conjugated IAA content per bud remained stable during the period of rapid flower growth until one day before anthesis.

AB - Dormant coffee (Coffea arabica L.) flower buds require water stress to stimulate regrowth. A xylem specific water-soluble dye, azosulfamide, was used to quantify water uptake of buds after their release from dormancy by water stress. In non-stressed flower buds, the rate of water uptake was generally slower and variable compared to stressed flower buds, where the rate of uptake tripled from 1 to 3 days after rewatering and preceded the doubling of fresh and dry weight of buds. Free, ester and amide IAA levels of developing flower buds were measured by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution technique with [13C6]IAA as an internal standard. Throughout development, the majority of IAA was present as amide IAA. The proportions of amide and free IAA increased one day after plants were released from water stress, and preceded the doubling of fresh and dry weight. Free and conjugated IAA content per bud remained stable during the period of rapid flower growth until one day before anthesis.

KW - Coffea arabica

KW - flower bud dormancy

KW - water stress

UR - http://www.scopus.com/inward/record.url?scp=0028155437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028155437&partnerID=8YFLogxK

U2 - 10.1007/BF00024674

DO - 10.1007/BF00024674

M3 - Article

AN - SCOPUS:0028155437

VL - 15

SP - 33

EP - 41

JO - Plant Growth Regulation

JF - Plant Growth Regulation

SN - 0167-6903

IS - 1

ER -