Endogenous IAA levels and development of coffee flower buds from dormancy to anthesis

Ursula K. Schuch, Anita N. Azarenko, Leslie H. Fuchigami

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Dormant coffee (Coffea arabica L.) flower buds require water stress to stimulate regrowth. A xylem specific water-soluble dye, azosulfamide, was used to quantify water uptake of buds after their release from dormancy by water stress. In non-stressed flower buds, the rate of water uptake was generally slower and variable compared to stressed flower buds, where the rate of uptake tripled from 1 to 3 days after rewatering and preceded the doubling of fresh and dry weight of buds. Free, ester and amide IAA levels of developing flower buds were measured by gas chromatography-mass spectrometry-selective ion monitoring using an isotope dilution technique with [13C6]IAA as an internal standard. Throughout development, the majority of IAA was present as amide IAA. The proportions of amide and free IAA increased one day after plants were released from water stress, and preceded the doubling of fresh and dry weight. Free and conjugated IAA content per bud remained stable during the period of rapid flower growth until one day before anthesis.

Original languageEnglish (US)
Pages (from-to)33-41
Number of pages9
JournalPlant Growth Regulation
Volume15
Issue number1
DOIs
StatePublished - Jul 1 1994
Externally publishedYes

Keywords

  • Coffea arabica
  • flower bud dormancy
  • water stress

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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