Endogenous interleukin 6 plays an obligatory role in interleukin 4-dependent human IgE synthesis

Donata Vercelli, H. H. Jabara, K. I. Arai, T. Yokota, R. S. Geha

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

The lymphokine interleukin (IL) 4 plays a crucial role in the regulation of IgE synthesis. In the present study, the cellular and cytokine requirements for the IL 4-dependent induction of IgE synthesis in humans were analyzed. Recombinant IL 4 could induce IgE synthesis by peripheral blood mononuclear cells and autologous T/B cell mixtures, but not by highly purified B cells. IgE induction by IL 4 was strongly decreased in monocyte-depleted peripheral blood mononuclear cells. These results show that the induction of IgE synthesis by recombinant IL 4 is T cell dependent and optimal in the presence of monocytes. IL 5 and IL 6, but not IL 2, IL 1 and tumor necrosis factor-α, strongly up-regulated the IL 4-dependent synthesis of IgE, with modest effects on cell proliferation. An anti-IL 6 polyclonal antibody strongly inhibited IL 4-driven IgE production. Endogenous IL 6 plays, therefore, an obligatory role in the IL 4-dependent induction of IgE. However, a combination of IL 4, IL 5 and IL 6 (with or without IL 1) at optimal concentrations could not induce IgE synthesis by purified normal B cells, indicating that cytokine-mediated signals, although essential, are not sufficient for the IL 4-dependent induction of IgE synthesis.

Original languageEnglish (US)
Pages (from-to)1419-1424
Number of pages6
JournalEuropean Journal of Immunology
Volume19
Issue number8
StatePublished - 1989
Externally publishedYes

Fingerprint

Interleukin-4
Immunoglobulin E
Interleukin-6
B-Lymphocytes
Interleukin-5
Interleukin-1
Monocytes
Blood Cells
Cytokines
Lymphokines
Interleukin-2
Tumor Necrosis Factor-alpha
Cell Proliferation
T-Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Endogenous interleukin 6 plays an obligatory role in interleukin 4-dependent human IgE synthesis. / Vercelli, Donata; Jabara, H. H.; Arai, K. I.; Yokota, T.; Geha, R. S.

In: European Journal of Immunology, Vol. 19, No. 8, 1989, p. 1419-1424.

Research output: Contribution to journalArticle

Vercelli, Donata ; Jabara, H. H. ; Arai, K. I. ; Yokota, T. ; Geha, R. S. / Endogenous interleukin 6 plays an obligatory role in interleukin 4-dependent human IgE synthesis. In: European Journal of Immunology. 1989 ; Vol. 19, No. 8. pp. 1419-1424.
@article{9a3aadc6821a4b38beb4f87be41c1faf,
title = "Endogenous interleukin 6 plays an obligatory role in interleukin 4-dependent human IgE synthesis",
abstract = "The lymphokine interleukin (IL) 4 plays a crucial role in the regulation of IgE synthesis. In the present study, the cellular and cytokine requirements for the IL 4-dependent induction of IgE synthesis in humans were analyzed. Recombinant IL 4 could induce IgE synthesis by peripheral blood mononuclear cells and autologous T/B cell mixtures, but not by highly purified B cells. IgE induction by IL 4 was strongly decreased in monocyte-depleted peripheral blood mononuclear cells. These results show that the induction of IgE synthesis by recombinant IL 4 is T cell dependent and optimal in the presence of monocytes. IL 5 and IL 6, but not IL 2, IL 1 and tumor necrosis factor-α, strongly up-regulated the IL 4-dependent synthesis of IgE, with modest effects on cell proliferation. An anti-IL 6 polyclonal antibody strongly inhibited IL 4-driven IgE production. Endogenous IL 6 plays, therefore, an obligatory role in the IL 4-dependent induction of IgE. However, a combination of IL 4, IL 5 and IL 6 (with or without IL 1) at optimal concentrations could not induce IgE synthesis by purified normal B cells, indicating that cytokine-mediated signals, although essential, are not sufficient for the IL 4-dependent induction of IgE synthesis.",
author = "Donata Vercelli and Jabara, {H. H.} and Arai, {K. I.} and T. Yokota and Geha, {R. S.}",
year = "1989",
language = "English (US)",
volume = "19",
pages = "1419--1424",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "8",

}

TY - JOUR

T1 - Endogenous interleukin 6 plays an obligatory role in interleukin 4-dependent human IgE synthesis

AU - Vercelli, Donata

AU - Jabara, H. H.

AU - Arai, K. I.

AU - Yokota, T.

AU - Geha, R. S.

PY - 1989

Y1 - 1989

N2 - The lymphokine interleukin (IL) 4 plays a crucial role in the regulation of IgE synthesis. In the present study, the cellular and cytokine requirements for the IL 4-dependent induction of IgE synthesis in humans were analyzed. Recombinant IL 4 could induce IgE synthesis by peripheral blood mononuclear cells and autologous T/B cell mixtures, but not by highly purified B cells. IgE induction by IL 4 was strongly decreased in monocyte-depleted peripheral blood mononuclear cells. These results show that the induction of IgE synthesis by recombinant IL 4 is T cell dependent and optimal in the presence of monocytes. IL 5 and IL 6, but not IL 2, IL 1 and tumor necrosis factor-α, strongly up-regulated the IL 4-dependent synthesis of IgE, with modest effects on cell proliferation. An anti-IL 6 polyclonal antibody strongly inhibited IL 4-driven IgE production. Endogenous IL 6 plays, therefore, an obligatory role in the IL 4-dependent induction of IgE. However, a combination of IL 4, IL 5 and IL 6 (with or without IL 1) at optimal concentrations could not induce IgE synthesis by purified normal B cells, indicating that cytokine-mediated signals, although essential, are not sufficient for the IL 4-dependent induction of IgE synthesis.

AB - The lymphokine interleukin (IL) 4 plays a crucial role in the regulation of IgE synthesis. In the present study, the cellular and cytokine requirements for the IL 4-dependent induction of IgE synthesis in humans were analyzed. Recombinant IL 4 could induce IgE synthesis by peripheral blood mononuclear cells and autologous T/B cell mixtures, but not by highly purified B cells. IgE induction by IL 4 was strongly decreased in monocyte-depleted peripheral blood mononuclear cells. These results show that the induction of IgE synthesis by recombinant IL 4 is T cell dependent and optimal in the presence of monocytes. IL 5 and IL 6, but not IL 2, IL 1 and tumor necrosis factor-α, strongly up-regulated the IL 4-dependent synthesis of IgE, with modest effects on cell proliferation. An anti-IL 6 polyclonal antibody strongly inhibited IL 4-driven IgE production. Endogenous IL 6 plays, therefore, an obligatory role in the IL 4-dependent induction of IgE. However, a combination of IL 4, IL 5 and IL 6 (with or without IL 1) at optimal concentrations could not induce IgE synthesis by purified normal B cells, indicating that cytokine-mediated signals, although essential, are not sufficient for the IL 4-dependent induction of IgE synthesis.

UR - http://www.scopus.com/inward/record.url?scp=0024440156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024440156&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 1419

EP - 1424

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 8

ER -