Endogenous levels of β-carotene in human buccal mucosa cells by reversed-phase high-performance liquid chromatography

Stephen A. Newcomb, Alison J. Culling-Berglund, Thomas P Davis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a reversed-phase high-performance liquid chromatographic assay for the measurement of low nanogram levels of β-carotene in a single sample of human buccal mucosa cells. The method includes a simple sonification step for cell disruption and release of the compounds into the supernatant. The limits of detection were 0.02, 0.02 and 0.07 ng/mg of protein for β-carotene, retinol and retinol palmitate, respectively. Two patient populations were analysed. Average endogenous levels for β-carotene normalized to protein were 0.25 ng/mg of protein (range 0.04-1.9 ng/mg, twelve patients). No evidence of endogenous retinol or retinol palmitate could be detected in the human samples. An oral dosing study of four normal individuals showed a wide variation of β-carotene uptake. This rapid and sensitive method will enable investigators to use the non-invasive technique of buccal mucosa cell harvesting to determine cellular depot levels of β-carotene in various patient populations.

Original languageEnglish (US)
Pages (from-to)47-58
Number of pages12
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume526
Issue numberC
DOIs
StatePublished - 1990

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Mouth Mucosa
High performance liquid chromatography
Reverse-Phase Chromatography
Carotenoids
High Pressure Liquid Chromatography
Vitamin A
Proteins
Population
Limit of Detection
Assays
Research Personnel
Mucous Membrane
Liquids
retinol palmitate

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Endogenous levels of β-carotene in human buccal mucosa cells by reversed-phase high-performance liquid chromatography. / Newcomb, Stephen A.; Culling-Berglund, Alison J.; Davis, Thomas P.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 526, No. C, 1990, p. 47-58.

Research output: Contribution to journalArticle

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