Endoglin and Alk5 regulate epithelial-mesenchymal transformation during cardiac valve formation

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Endoglin is an accessory receptor for TGFβ and can associate with Alk5 or Alk2. Although prior studies indicated that endoglin and Alk5 were not directly involved in epithelial-mesenchymal transformation (EMT) in the heart, the expression pattern of endoglin prompted a re-examination. We here show that loss of endoglin expression mediated by either antisense DNA or siRNA results in a direct perturbation of EMT and reduced expression of EMT markers including slug, runx2, RhoA, and latrophilin-2. An examination of BrdU incorporation shows that, while endoglin regulates proliferation at an early stage, reduced endothelial cell proliferation does not account for the loss of mesenchyme. As Alk5 interacts with endoglin, we utilized siRNA and a specific inhibitor, HTS466284 (HTS), to perturb this receptor as well. Alk5 inhibition produced similar effects to the inhibition of endoglin. There was a reduction in mesenchymal cell formation and loss of EMT marker expression similar to that seen with endoglin. Alk5 kinase inhibition produced a similar loss of EMT marker expression but showed a contrasting upregulation of the proliferation and remodeling markers, Cyclin B2 and β-catenin. Alk5 and endoglin both mediate endothelial cell proliferation in younger explants but, by stage 16, loss of endoglin no longer alters proliferation rates. These data show that both Alk5 and endoglin are directly involved in the process of EMT, that they interact with both TGFβ-regulated activation and invasion pathways and that the roles of these receptors change during cardiac development.

Original languageEnglish (US)
Pages (from-to)420-432
Number of pages13
JournalDevelopmental Biology
Volume304
Issue number1
DOIs
StatePublished - Apr 1 2007

Fingerprint

Epithelial-Mesenchymal Transition
Heart Valves
Small Interfering RNA
Cyclin B2
Endoglin
Endothelial Cells
Antisense DNA
Cell Proliferation
Catenins
Gastropoda
Mesoderm
Bromodeoxyuridine
Phosphotransferases
Up-Regulation

Keywords

  • EMT
  • Runx2
  • TGFbeta 3
  • TRβRI

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Endoglin and Alk5 regulate epithelial-mesenchymal transformation during cardiac valve formation. / Mercado-Pimentel, Melania; Hubbard, Antony D.; Runyan, Raymond B.

In: Developmental Biology, Vol. 304, No. 1, 01.04.2007, p. 420-432.

Research output: Contribution to journalArticle

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