Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model

Lida P. Hariri, Alesandre R. Tumlinson, David G. Besselsen, Urs Utzinger, Eugene W. Gerner, Jennifer K. Barton

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Background and Objectives: The diagnostic feasibility of optical coherence tomography (OCT) and laser-induced fluorescence (LIF) have been evaluated for human colorectal cancer. This study applies these technologies to a murine model of colorectal adenoma. Study Design/Materials and Methods: The lower colon of 10 ApcMin and two C57BL/6J mice was surveyed over five 4-week intervals using a prototype 2.0 mm diameter OCT-LIF endoscope-based system. Four categories were histologically classified: control C57BL/6J, adenomatous, non-diseased regions of adenomatous, and non-diseased ApcMin. OCT images were compared to histology. Spectra from the four categories were compared via the Student's t-test. Results: Three ApcMin and two control mice completed the study. One adenoma was histologically identified; OCT visualized mucosal thickening/abnormal mass development over the imaging timepoints. LIF spectral comparisons revealed decreased 405 nm intensity and the presence of a peak at 680 nm in the adenomatous ApcMin. Conclusions: These preliminary data indicate endoscopic OCT-LIF has the potential to identify colorectal adenomas in murine models.

Original languageEnglish (US)
Pages (from-to)305-313
Number of pages9
JournalLasers in Surgery and Medicine
Volume38
Issue number4
DOIs
StatePublished - Apr 1 2006

Keywords

  • Animal imaging
  • Animal models of carcinogenesis
  • Apc
  • Colorectal cancer
  • Gastrointestinal cancer
  • Imaging of tumor progression/metastasis

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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