Endoscopic spectral domain optical coherence tomography of murine colonic morphology to determine effectiveness of chemopreventive and chemotherapeutic agents in colorectal cancer

Susan LeGendre-McGhee, Photini F.S. Rice, R. Andrew Wall, Justin Klein, Amber Luttman, Kyle Sprute, Eugene Gerner, Jennifer K. Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical coherence tomography (OCT) is a minimally-invasive imaging modality capable of tracking the development of individual colonic adenomas. As such, OCT can be used to evaluate the mechanisms and effectiveness of chemopreventive and chemotherapeutic agents in colorectal cancer models. The data presented here represent part of a larger study evaluating α-difluoromethylornithine (DFMO) and Sulindac as chemopreventive and chemotherapeutic agents using mice treated with the carcinogen azoxymethane (AOM). 27 A/J mice were included in the chemoprevention study, subdivided into four treatment groups (No Drug, DFMO, Sulindac, DFMO/Sulindac). 30 mm lateral images of each colon at eight different rotations were obtained at five different time points using a 2 mm diameter spectral domain OCT endoscopy system centered at 890 nm with 3.5 μm axial resolution in air and 5 μm lateral resolution. Images were visually analyzed to determine number and size of adenomas. Gross photos of the excised colons and histology provided gold standard confirmation of the final imaging time point. Preliminary results show that 100% of mice in the No Drug group developed adenomas over the course of the chemoprevention study. Incidence was reduced to 71.43% in mice given DFMO, 85.71% for Sulindac and 0% for DFMO/Sulindac. Discrete adenoma size did not vary significantly between experimental groups. Additional experiments are currently under way to verify these results and evaluate DFMO and Sulindac for chemotherapeutic applications.

Original languageEnglish (US)
Title of host publicationEndoscopic Microscopy VII
DOIs
StatePublished - Mar 26 2012
EventEndoscopic Microscopy VII - San Francisco, CA, United States
Duration: Jan 22 2012Jan 23 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8217
ISSN (Print)1605-7422

Other

OtherEndoscopic Microscopy VII
CountryUnited States
CitySan Francisco, CA
Period1/22/121/23/12

Keywords

  • Chemoprevention
  • Colorectal cancer
  • Optical coherence tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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