Endothelial cell monolayer dysfunction caused by oxidized low density lipoprotein: Attenuation by oleic acid

R. J. Karman, J. G.N. Garcia, C. M. Hart

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Oleic acid (18:1) may exert beneficial effects on the pathogenesis of vascular disease by a variety of mechanisms. To determine if 18:1 exerts direct protective effects on vascular endothelial cells, porcine pulmonary artery endothelial cells (PAEC) were supplemented with 0.1 mM 18:1, γ-linolenic acid (18:3), or ethanol vehicle (ETOH) prior to treatment with low density lipoprotein (LDL), or Cu2+-oxidized (LDL (OXLDL). Treatment with neither LDL nor OXLDL (100 μg protein/ml) for 24-48 h caused PAEC cytotoxicity, whereas OXLDL, but not (LDL, caused derangements in PAEC actin microfilament architecture and monolayer barrier dysfunction. Supplementation with 18:1, but not 18:3, attenuated derangements caused by OXLDL and lysophosphatidylcholine, a component of OXLDL. These results demonstrate that monounsaturated fatty acids directly alter the response of vascular endothelial cells to OXLDL and may retard the atherosclerotic process by decreasing the efflux of macromolecules (e.g. (LDL) into the vessel wall.

Original languageEnglish (US)
Pages (from-to)345-353
Number of pages9
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume56
Issue number5
DOIs
StatePublished - May 1997
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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