Endothelial cells are a replicative niche for entry of Toxoplasma gondii to the central nervous system

Christoph Konradt, Norikiyo Ueno, David A. Christian, Jonathan H. Delong, Gretchen Harms Pritchard, Jasmin Herz, David J. Bzik, Anita A Koshy, Dorian B. McGavern, Melissa B. Lodoen, Christopher A. Hunter

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

An important function of the blood-brain barrier is to exclude pathogens from the central nervous system, but some microorganisms benefit from the ability to enter this site. It has been proposed that Toxoplasma gondii can cross biological barriers as a motile extracellular form that uses transcellular or paracellular migration, or by infecting a host cell that then crosses the blood-brain barrier. Unexpectedly, analysis of acutely infected mice revealed significant numbers of free parasites in the blood and the presence of infected endothelial cells in the brain vasculature. The use of diverse transgenic parasites combined with reporter mice and intravital imaging demonstrated that replication in and lysis of endothelial cells precedes invasion of the central nervous system, and highlight a novel mechanism for parasite entry to the central nervous system.

Original languageEnglish (US)
Article number16001
JournalNature Microbiology
Volume1
Issue number3
DOIs
StatePublished - Feb 15 2016

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology (medical)
  • Cell Biology
  • Genetics

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    Konradt, C., Ueno, N., Christian, D. A., Delong, J. H., Pritchard, G. H., Herz, J., Bzik, D. J., Koshy, A. A., McGavern, D. B., Lodoen, M. B., & Hunter, C. A. (2016). Endothelial cells are a replicative niche for entry of Toxoplasma gondii to the central nervous system. Nature Microbiology, 1(3), [16001]. https://doi.org/10.1038/nmicrobiol.2016.1