Endothelial nitric oxide synthase tagging single nucleotide polymorphisms and recovery from aneurysmal subarachnoid hemorrhage

Sheila Alexander, Samuel Poloyac, Leslie Hoffman, Matthew J Gallek, Ren Dianxu Ren, Jeffrey Balzer, Amin Kassam, Yvette Conley

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aneurysmal subarachnoid hemorrhage (SAH) is a hemorrhagic stroke subtype with a poor recovery profile. Cerebral vasospasm (CV), a narrowing of the cerebral vasculature, significantly contributes to the poor recovery profile. Variation in the endothelial nitric oxide (NO) synthase (eNOS) gene has been implicated in CV and outcome after SAH. The purpose of this project was to explore the potential association between three eNOS tagging single nucleotide polymorphisms (SNPs) and recovery from SAH. We included 195 participants with a diagnosis of SAH and DNA and 6-month outcome data available but without preexisting neurologic disease/deficit. Genotyping was performed using an ABI Prism 7000 Sequence Detection System and TaqMan assays. CV was verified by cerebral angiogram independently read by a neurosurgeon on 118 participants. Modified Rankin Scores (MRS) and Glasgow Outcome Scale (GOS) scores were collected 6 months posthemorrhage. Data were analyzed using descriptive statistics, analysis of variance (ANOVA) and chi-square analysis as appropriate. The sample was primarily female (n = 147; 75.4%) and White (n = 178; 91.3%) with a mean age of 54.6 years. Of the participants with CV data, 56 (47.5%) developed CV within 14 days of SAH. None of the SNPs individually were associated with CV presence; however, a combination of the three variant SNPs was significantly associated with CV (p =.017). Only one SNP (rs1799983, variant allele) was associated with worse 6-month GOS scores (p <.001) and MRS (p <.001). These data indicate that the eNOS gene plays a role in the response to SAH, which may be explained by an influence on CV.

Original languageEnglish (US)
Pages (from-to)42-52
Number of pages11
JournalBiological Research for Nursing
Volume11
Issue number1
DOIs
StatePublished - Jul 2009

Fingerprint

Intracranial Vasospasm
Nitric Oxide Synthase Type III
Subarachnoid Hemorrhage
Single Nucleotide Polymorphism
Glasgow Outcome Scale
Preexisting Condition Coverage
Neurologic Manifestations
Nervous System Diseases
Genes
Analysis of Variance
Angiography
Stroke
Alleles
DNA

Keywords

  • Cerebral vasospasm
  • Genotype
  • Nitric oxide
  • Outcome
  • Subarachnoid hemorrhage

ASJC Scopus subject areas

  • Research and Theory

Cite this

Endothelial nitric oxide synthase tagging single nucleotide polymorphisms and recovery from aneurysmal subarachnoid hemorrhage. / Alexander, Sheila; Poloyac, Samuel; Hoffman, Leslie; Gallek, Matthew J; Dianxu Ren, Ren; Balzer, Jeffrey; Kassam, Amin; Conley, Yvette.

In: Biological Research for Nursing, Vol. 11, No. 1, 07.2009, p. 42-52.

Research output: Contribution to journalArticle

Alexander, Sheila ; Poloyac, Samuel ; Hoffman, Leslie ; Gallek, Matthew J ; Dianxu Ren, Ren ; Balzer, Jeffrey ; Kassam, Amin ; Conley, Yvette. / Endothelial nitric oxide synthase tagging single nucleotide polymorphisms and recovery from aneurysmal subarachnoid hemorrhage. In: Biological Research for Nursing. 2009 ; Vol. 11, No. 1. pp. 42-52.
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abstract = "Aneurysmal subarachnoid hemorrhage (SAH) is a hemorrhagic stroke subtype with a poor recovery profile. Cerebral vasospasm (CV), a narrowing of the cerebral vasculature, significantly contributes to the poor recovery profile. Variation in the endothelial nitric oxide (NO) synthase (eNOS) gene has been implicated in CV and outcome after SAH. The purpose of this project was to explore the potential association between three eNOS tagging single nucleotide polymorphisms (SNPs) and recovery from SAH. We included 195 participants with a diagnosis of SAH and DNA and 6-month outcome data available but without preexisting neurologic disease/deficit. Genotyping was performed using an ABI Prism 7000 Sequence Detection System and TaqMan assays. CV was verified by cerebral angiogram independently read by a neurosurgeon on 118 participants. Modified Rankin Scores (MRS) and Glasgow Outcome Scale (GOS) scores were collected 6 months posthemorrhage. Data were analyzed using descriptive statistics, analysis of variance (ANOVA) and chi-square analysis as appropriate. The sample was primarily female (n = 147; 75.4{\%}) and White (n = 178; 91.3{\%}) with a mean age of 54.6 years. Of the participants with CV data, 56 (47.5{\%}) developed CV within 14 days of SAH. None of the SNPs individually were associated with CV presence; however, a combination of the three variant SNPs was significantly associated with CV (p =.017). Only one SNP (rs1799983, variant allele) was associated with worse 6-month GOS scores (p <.001) and MRS (p <.001). These data indicate that the eNOS gene plays a role in the response to SAH, which may be explained by an influence on CV.",
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