Endothelial transdifferentiated phenotype and cell-cycle kinetics of AIDS- associated Kaposi sarcoma cells

D. L. Way, Marlys H Witte, M. Fiala, G. Ramirez, Raymond B Nagle, Michael J Bernas, M. Dictor, P. Borgs, C. L. Witte

Research output: Contribution to journalArticle

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Abstract

The nature of Kaposi sarcoma (KS) (vascular malignancy vs. discordant angiogenesis) and lineage of the progenitor cell remain unclear. Therefore, AIDS-KS enzyme isolate cultures were prepared from excised skin lesions. Endothelial marker positivity for Factor VIII related antigen (F8RAg), Ulex europaeus agglutinin (UEA), and angiotensin-converting enzyme (ACE) were determined by fluorescence microscopy (FM) and flow cytometry (FCM). DNA cell-cycle analysis was performed using FCM. KS lesions showed large thick- walled channels (F8RAg and UEA strongly +), narrow vascular slits and thin- walled lakes (F8RAg and UEA weakly +), and non-prominent spindle cells (F8RAg and UEA almost uniformly negative). KS cultures yielded heterogenous populations of spindle, stellate, and flattened endothelial-like cells, displaying positivity for F8RAg (64±3%; mean±SE), UEA (40±9%), and ACE (81±9%). When injected subcutaneously in the nude mouse these cells failed to produce tumors. During contact inhibition induced quiescence, KS cultures exhibited a high G2M (18±3%) compared to non-KS (7±4%; p<0.04), evidence of an altered proliferative potential consistent with a transdifferentiated or transformed phenotype.

Original languageEnglish (US)
Pages (from-to)79-89
Number of pages11
JournalLymphology
Volume26
Issue number2
StatePublished - 1993

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Ulex
Agglutinins
Kaposi's Sarcoma
Cell Cycle
Acquired Immunodeficiency Syndrome
Phenotype
Peptidyl-Dipeptidase A
Blood Vessels
Flow Cytometry
Contact Inhibition
von Willebrand Factor
Lakes
Fluorescence Microscopy
Nude Mice
Sarcoma
Neoplasms
Stem Cells
Endothelial Cells
Skin
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Endothelial transdifferentiated phenotype and cell-cycle kinetics of AIDS- associated Kaposi sarcoma cells. / Way, D. L.; Witte, Marlys H; Fiala, M.; Ramirez, G.; Nagle, Raymond B; Bernas, Michael J; Dictor, M.; Borgs, P.; Witte, C. L.

In: Lymphology, Vol. 26, No. 2, 1993, p. 79-89.

Research output: Contribution to journalArticle

Way, DL, Witte, MH, Fiala, M, Ramirez, G, Nagle, RB, Bernas, MJ, Dictor, M, Borgs, P & Witte, CL 1993, 'Endothelial transdifferentiated phenotype and cell-cycle kinetics of AIDS- associated Kaposi sarcoma cells', Lymphology, vol. 26, no. 2, pp. 79-89.
Way, D. L. ; Witte, Marlys H ; Fiala, M. ; Ramirez, G. ; Nagle, Raymond B ; Bernas, Michael J ; Dictor, M. ; Borgs, P. ; Witte, C. L. / Endothelial transdifferentiated phenotype and cell-cycle kinetics of AIDS- associated Kaposi sarcoma cells. In: Lymphology. 1993 ; Vol. 26, No. 2. pp. 79-89.
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AU - Bernas, Michael J

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