Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study

M. Kriat, J. Fantini, J. Vion-Dury, S. Confort-Gouny, Jean-Philippe Galons, P. J. Cozzone

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Insulin-secreting cells (RINm5F) have successfully been grown on a large scale on poly-l-lysine coated-polystyrene microcarriers, providing a high cell number in a restricted volume under conditions that respect the metabolic integrity of these anchorage-dependent cells. The energetic metabolism of the perfused cells hae been followed non-invasively by phosphorus-31 nuclear magnetic resonance spectroscopy. Glucose starvation induced a rapid decrease in nucleoside triphosphates (mainly ATP) pools, correlated with an increase in Pi level. The initial ATP level was rapidly recovered when the cells were refed with glucose or wth mannose, but not with galactose, even after 2 h of perfusion. These differential effects of hexoses on energetic metabolism might be related to their various insulin-release actions on tumor islet cells.

Original languageEnglish (US)
Pages (from-to)949-955
Number of pages7
JournalBiochimie
Volume74
Issue number9-10
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Insulinoma
Mannose
Galactose
Metabolism
Phosphorus
Rats
Adenosine Triphosphate
Nuclear magnetic resonance
Insulin
Glucose
Hexoses
Polystyrenes
Nucleosides
Nuclear magnetic resonance spectroscopy
Islet Cell Adenoma
Lysine
Tumors
Insulin-Secreting Cells
Starvation
Magnetic Resonance Spectroscopy

Keywords

  • P NMR spectroscopy/ RINm5F cells/ microcarriers culture/ hexose metabolism

ASJC Scopus subject areas

  • Biochemistry

Cite this

Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study. / Kriat, M.; Fantini, J.; Vion-Dury, J.; Confort-Gouny, S.; Galons, Jean-Philippe; Cozzone, P. J.

In: Biochimie, Vol. 74, No. 9-10, 1992, p. 949-955.

Research output: Contribution to journalArticle

Kriat, M. ; Fantini, J. ; Vion-Dury, J. ; Confort-Gouny, S. ; Galons, Jean-Philippe ; Cozzone, P. J. / Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study. In: Biochimie. 1992 ; Vol. 74, No. 9-10. pp. 949-955.
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AU - Cozzone, P. J.

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