Enhanced imaging of biomolecules with electron beam deposited tips for scanning force microscopy

Frederic Zenhausern, M. Adrian, B. Ten Heggeler-Bordier, F. Ardizzoni, P. Descouts

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Tip/sample interaction on the scanning force microscope (SFM) is a particularly difficult problem with biological materials. One major factor affecting image quality is the tip shape. Improved electron beam induced deposition technique with a scanning electron microscope (SEM) was used for the reproducible fabrication of carbon sharp tips on the end of commercially available silicon nitride cantilevers for scanning force microscopy. By aligning a fine focused beam of 20 nm diameter directly down the axis of the pyramidal tip at electron energy of 20 kV, carbon deposits grow with full cone angle of about 25°, cone length of 2 μm, and radii of curvature down to 10 nm, making these e-beam tips suitable for biomolecules imaging. The tip dimensions also were controlled by adjusting the beam parameters. Three different types of SFM tips were used to image tobacco mosaic virus (TMV). Conventional pyramidal tips appeared generally worse for imaging helical particles of TMV than SEM-deposited tips which were found more robust than commercially available conical tips. The use of sharper tips for SFM imaging of protein DNA revealed a 25% improvement in lateral resolution.

Original languageEnglish (US)
Pages (from-to)7232-7237
Number of pages6
JournalJournal of Applied Physics
Volume73
Issue number11
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

electron beams
microscopy
scanning
tobacco
microscopes
viruses
cones
electron microscopes
carbon
silicon nitrides
deoxyribonucleic acid
adjusting
deposits
curvature
electron energy
proteins
fabrication
radii

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Enhanced imaging of biomolecules with electron beam deposited tips for scanning force microscopy. / Zenhausern, Frederic; Adrian, M.; Ten Heggeler-Bordier, B.; Ardizzoni, F.; Descouts, P.

In: Journal of Applied Physics, Vol. 73, No. 11, 1993, p. 7232-7237.

Research output: Contribution to journalArticle

Zenhausern, Frederic ; Adrian, M. ; Ten Heggeler-Bordier, B. ; Ardizzoni, F. ; Descouts, P. / Enhanced imaging of biomolecules with electron beam deposited tips for scanning force microscopy. In: Journal of Applied Physics. 1993 ; Vol. 73, No. 11. pp. 7232-7237.
@article{3c1d14e324ae443ab9283f912c6ff032,
title = "Enhanced imaging of biomolecules with electron beam deposited tips for scanning force microscopy",
abstract = "Tip/sample interaction on the scanning force microscope (SFM) is a particularly difficult problem with biological materials. One major factor affecting image quality is the tip shape. Improved electron beam induced deposition technique with a scanning electron microscope (SEM) was used for the reproducible fabrication of carbon sharp tips on the end of commercially available silicon nitride cantilevers for scanning force microscopy. By aligning a fine focused beam of 20 nm diameter directly down the axis of the pyramidal tip at electron energy of 20 kV, carbon deposits grow with full cone angle of about 25°, cone length of 2 μm, and radii of curvature down to 10 nm, making these e-beam tips suitable for biomolecules imaging. The tip dimensions also were controlled by adjusting the beam parameters. Three different types of SFM tips were used to image tobacco mosaic virus (TMV). Conventional pyramidal tips appeared generally worse for imaging helical particles of TMV than SEM-deposited tips which were found more robust than commercially available conical tips. The use of sharper tips for SFM imaging of protein DNA revealed a 25{\%} improvement in lateral resolution.",
author = "Frederic Zenhausern and M. Adrian and {Ten Heggeler-Bordier}, B. and F. Ardizzoni and P. Descouts",
year = "1993",
doi = "10.1063/1.354010",
language = "English (US)",
volume = "73",
pages = "7232--7237",
journal = "Journal of Applied Physics",
issn = "0021-8979",
publisher = "American Institute of Physics Publising LLC",
number = "11",

}

TY - JOUR

T1 - Enhanced imaging of biomolecules with electron beam deposited tips for scanning force microscopy

AU - Zenhausern, Frederic

AU - Adrian, M.

AU - Ten Heggeler-Bordier, B.

AU - Ardizzoni, F.

AU - Descouts, P.

PY - 1993

Y1 - 1993

N2 - Tip/sample interaction on the scanning force microscope (SFM) is a particularly difficult problem with biological materials. One major factor affecting image quality is the tip shape. Improved electron beam induced deposition technique with a scanning electron microscope (SEM) was used for the reproducible fabrication of carbon sharp tips on the end of commercially available silicon nitride cantilevers for scanning force microscopy. By aligning a fine focused beam of 20 nm diameter directly down the axis of the pyramidal tip at electron energy of 20 kV, carbon deposits grow with full cone angle of about 25°, cone length of 2 μm, and radii of curvature down to 10 nm, making these e-beam tips suitable for biomolecules imaging. The tip dimensions also were controlled by adjusting the beam parameters. Three different types of SFM tips were used to image tobacco mosaic virus (TMV). Conventional pyramidal tips appeared generally worse for imaging helical particles of TMV than SEM-deposited tips which were found more robust than commercially available conical tips. The use of sharper tips for SFM imaging of protein DNA revealed a 25% improvement in lateral resolution.

AB - Tip/sample interaction on the scanning force microscope (SFM) is a particularly difficult problem with biological materials. One major factor affecting image quality is the tip shape. Improved electron beam induced deposition technique with a scanning electron microscope (SEM) was used for the reproducible fabrication of carbon sharp tips on the end of commercially available silicon nitride cantilevers for scanning force microscopy. By aligning a fine focused beam of 20 nm diameter directly down the axis of the pyramidal tip at electron energy of 20 kV, carbon deposits grow with full cone angle of about 25°, cone length of 2 μm, and radii of curvature down to 10 nm, making these e-beam tips suitable for biomolecules imaging. The tip dimensions also were controlled by adjusting the beam parameters. Three different types of SFM tips were used to image tobacco mosaic virus (TMV). Conventional pyramidal tips appeared generally worse for imaging helical particles of TMV than SEM-deposited tips which were found more robust than commercially available conical tips. The use of sharper tips for SFM imaging of protein DNA revealed a 25% improvement in lateral resolution.

UR - http://www.scopus.com/inward/record.url?scp=0007170012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0007170012&partnerID=8YFLogxK

U2 - 10.1063/1.354010

DO - 10.1063/1.354010

M3 - Article

AN - SCOPUS:0007170012

VL - 73

SP - 7232

EP - 7237

JO - Journal of Applied Physics

JF - Journal of Applied Physics

SN - 0021-8979

IS - 11

ER -