Enhanced release of insulin by prostaglandins in isolated pancreatic islets

David G Johnson, W. Y. Fujimoto, R. H. Williams

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The effects of several prostaglandins on insulin release were studied during incubation of isolated pancreatic islets from the rat. The glucose stimulated release of insulin into medium containing 300 mg % glucose and 1.0 mM theophylline was increased approximately twofold by PGE1, PGE2, and PGF(2α). The effect of PGE1 was apparent at doses as low as 10-8 M. and progressively increased with concentrations up to 10-5 M. PGA1 did not affect insulin release. Inhibition of insulin release by norepinephrine (10-5 M) was partially reversed by both PGE1 and PGE2. None of the prostaglandins affected the small basal release of insulin into medium containing 30 mg % glucose. PGE1 (10-5 M) increased the accumulation of cyclic AMP formed from labeled precursor by pancreatic islets incubated in media containing 10 mM theophylline and either 30 or 300 mg % glucose. These results indicate that prostaglandins can enhance the release of insulin from beta cells in response to glucose. This action is antagonistic to the inhibitory effect of norepinephrine on insulin release and may be mediated through effects on adenyl cyclase activity.

Original languageEnglish (US)
Pages (from-to)658-663
Number of pages6
JournalDiabetes
Volume22
Issue number9
StatePublished - 1973
Externally publishedYes

Fingerprint

Islets of Langerhans
Prostaglandins
Insulin
Alprostadil
Glucose
Theophylline
Dinoprostone
Norepinephrine
Prostaglandins F
Adenylyl Cyclases
Cyclic AMP

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Johnson, D. G., Fujimoto, W. Y., & Williams, R. H. (1973). Enhanced release of insulin by prostaglandins in isolated pancreatic islets. Diabetes, 22(9), 658-663.

Enhanced release of insulin by prostaglandins in isolated pancreatic islets. / Johnson, David G; Fujimoto, W. Y.; Williams, R. H.

In: Diabetes, Vol. 22, No. 9, 1973, p. 658-663.

Research output: Contribution to journalArticle

Johnson, DG, Fujimoto, WY & Williams, RH 1973, 'Enhanced release of insulin by prostaglandins in isolated pancreatic islets', Diabetes, vol. 22, no. 9, pp. 658-663.
Johnson, David G ; Fujimoto, W. Y. ; Williams, R. H. / Enhanced release of insulin by prostaglandins in isolated pancreatic islets. In: Diabetes. 1973 ; Vol. 22, No. 9. pp. 658-663.
@article{12bb379c39494cddbe8d54ad909297cc,
title = "Enhanced release of insulin by prostaglandins in isolated pancreatic islets",
abstract = "The effects of several prostaglandins on insulin release were studied during incubation of isolated pancreatic islets from the rat. The glucose stimulated release of insulin into medium containing 300 mg {\%} glucose and 1.0 mM theophylline was increased approximately twofold by PGE1, PGE2, and PGF(2α). The effect of PGE1 was apparent at doses as low as 10-8 M. and progressively increased with concentrations up to 10-5 M. PGA1 did not affect insulin release. Inhibition of insulin release by norepinephrine (10-5 M) was partially reversed by both PGE1 and PGE2. None of the prostaglandins affected the small basal release of insulin into medium containing 30 mg {\%} glucose. PGE1 (10-5 M) increased the accumulation of cyclic AMP formed from labeled precursor by pancreatic islets incubated in media containing 10 mM theophylline and either 30 or 300 mg {\%} glucose. These results indicate that prostaglandins can enhance the release of insulin from beta cells in response to glucose. This action is antagonistic to the inhibitory effect of norepinephrine on insulin release and may be mediated through effects on adenyl cyclase activity.",
author = "Johnson, {David G} and Fujimoto, {W. Y.} and Williams, {R. H.}",
year = "1973",
language = "English (US)",
volume = "22",
pages = "658--663",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association Inc.",
number = "9",

}

TY - JOUR

T1 - Enhanced release of insulin by prostaglandins in isolated pancreatic islets

AU - Johnson, David G

AU - Fujimoto, W. Y.

AU - Williams, R. H.

PY - 1973

Y1 - 1973

N2 - The effects of several prostaglandins on insulin release were studied during incubation of isolated pancreatic islets from the rat. The glucose stimulated release of insulin into medium containing 300 mg % glucose and 1.0 mM theophylline was increased approximately twofold by PGE1, PGE2, and PGF(2α). The effect of PGE1 was apparent at doses as low as 10-8 M. and progressively increased with concentrations up to 10-5 M. PGA1 did not affect insulin release. Inhibition of insulin release by norepinephrine (10-5 M) was partially reversed by both PGE1 and PGE2. None of the prostaglandins affected the small basal release of insulin into medium containing 30 mg % glucose. PGE1 (10-5 M) increased the accumulation of cyclic AMP formed from labeled precursor by pancreatic islets incubated in media containing 10 mM theophylline and either 30 or 300 mg % glucose. These results indicate that prostaglandins can enhance the release of insulin from beta cells in response to glucose. This action is antagonistic to the inhibitory effect of norepinephrine on insulin release and may be mediated through effects on adenyl cyclase activity.

AB - The effects of several prostaglandins on insulin release were studied during incubation of isolated pancreatic islets from the rat. The glucose stimulated release of insulin into medium containing 300 mg % glucose and 1.0 mM theophylline was increased approximately twofold by PGE1, PGE2, and PGF(2α). The effect of PGE1 was apparent at doses as low as 10-8 M. and progressively increased with concentrations up to 10-5 M. PGA1 did not affect insulin release. Inhibition of insulin release by norepinephrine (10-5 M) was partially reversed by both PGE1 and PGE2. None of the prostaglandins affected the small basal release of insulin into medium containing 30 mg % glucose. PGE1 (10-5 M) increased the accumulation of cyclic AMP formed from labeled precursor by pancreatic islets incubated in media containing 10 mM theophylline and either 30 or 300 mg % glucose. These results indicate that prostaglandins can enhance the release of insulin from beta cells in response to glucose. This action is antagonistic to the inhibitory effect of norepinephrine on insulin release and may be mediated through effects on adenyl cyclase activity.

UR - http://www.scopus.com/inward/record.url?scp=0015804165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015804165&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 658

EP - 663

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 9

ER -