Enhanced salt stress tolerance of rice plants expressing a vacuolar H +-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel

Niranjan Baisakh, Mangu V. Ramanarao, Kanniah Rajasekaran, Prasanta Subudhi, Jaroslav Janda, David W Galbraith, Cheryl Vanier, Andy Pereira

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The physiological role of a vacuolar ATPase subunit c1 (SaVHAc1) from a halophyte grass Spartina alterniflora was studied through its expression in rice. The SaVHAc1-expressing plants showed enhanced tolerance to salt stress than the wild-type plants, mainly through adjustments in early stage and preparatory physiological responses. In addition to the increased accumulation of its own transcript, SaVHAc1 expression led to increased accumulation of messages of other native genes in rice, especially those involved in cation transport and ABA signalling. The SaVHAc1-expressing plants maintained higher relative water content under salt stress through early stage closure of the leaf stoma and reduced stomata density. The increased K +/Na + ratio and other cations established an ion homoeostasis in SaVHAc1-expressing plants to protect the cytosol from toxic Na + and thereby maintained higher chlorophyll retention than the WT plants under salt stress. Besides, the role of SaVHAc1 in cell wall expansion and maintenance of net photosynthesis was implicated by comparatively higher root and leaf growth and yield of rice expressing SaVHAc1 over WT under salt stress. The study indicated that the genes contributing toward natural variation in grass halophytes could be effectively manipulated for improving salt tolerance of field crops within related taxa.

Original languageEnglish (US)
Pages (from-to)453-464
Number of pages12
JournalPlant Biotechnology Journal
Volume10
Issue number4
DOIs
StatePublished - May 2012

Fingerprint

Vacuolar Proton-Translocating ATPases
Salt-Tolerance
H-transporting ATP synthase
Spartina alterniflora
Poaceae
stress tolerance
salt stress
grasses
rice
Salts
Genes
genes
stomata
Cations
cations
Salt-Tolerant Plants
halophytes
Poisons
Photosynthesis
Chlorophyll

Keywords

  • Gene expression
  • Halophyte
  • Rice
  • Salt tolerance
  • Vacuolar ATPase

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Enhanced salt stress tolerance of rice plants expressing a vacuolar H +-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel. / Baisakh, Niranjan; Ramanarao, Mangu V.; Rajasekaran, Kanniah; Subudhi, Prasanta; Janda, Jaroslav; Galbraith, David W; Vanier, Cheryl; Pereira, Andy.

In: Plant Biotechnology Journal, Vol. 10, No. 4, 05.2012, p. 453-464.

Research output: Contribution to journalArticle

Baisakh, Niranjan ; Ramanarao, Mangu V. ; Rajasekaran, Kanniah ; Subudhi, Prasanta ; Janda, Jaroslav ; Galbraith, David W ; Vanier, Cheryl ; Pereira, Andy. / Enhanced salt stress tolerance of rice plants expressing a vacuolar H +-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel. In: Plant Biotechnology Journal. 2012 ; Vol. 10, No. 4. pp. 453-464.
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