Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge

F. J. Cervantes, T. Duong-Dac, K. Roest, A. D L Akkermans, G. Lettinga, James A Field

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The capacity of an anaerobic granular sludge for serving as an immobilizing mechanism for quinone-respiring bacteria was evaluated. The inoculum was continuously fed with a basal medium containing the humic model compound, anthraquinone-2,6-disulfonate (AQDS), as a terminal electron acceptor. Complete reduction of AQDS was achieved by the granular sludge for a prolonged period in an anaerobic bioreactor provided with a mixture of volatile fatty acids as a substrate. Phylogenetic analysis revealed the enrichment and immobilization of AQDS-respiring bacteria appearing as dominant organisms in the microbial population of the AQDS-supplemented reactor, compared to a reactor control operated under methanogenic conditions. The consistent quinone-reducing capacity observed in the consortium indicates that it is feasible to apply quinone-reducing microorganisms in continuous bioreactors and this ability can potentially be important in wastewaters rich in humic substances. The quinone reducing activity could also be applied to accelerate the conversion of xenobiotics susceptible to reductive biotransformations such as azo dyes and polychlorinated compounds in continuous bioreactors.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalWater Science and Technology
Volume48
Issue number6
StatePublished - 2003

Fingerprint

Bioreactors
bioreactor
immobilization
Bacteria
sludge
bacterium
Volatile fatty acids
Azo dyes
biotransformation
xenobiotics
humic substance
Microorganisms
dye
Wastewater
fatty acid
microorganism
phylogenetics
wastewater
substrate
electron

Keywords

  • AQDS
  • Immobilization
  • Quinones
  • UASB
  • Xenobiotics

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Cervantes, F. J., Duong-Dac, T., Roest, K., Akkermans, A. D. L., Lettinga, G., & Field, J. A. (2003). Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge. Water Science and Technology, 48(6), 9-16.

Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge. / Cervantes, F. J.; Duong-Dac, T.; Roest, K.; Akkermans, A. D L; Lettinga, G.; Field, James A.

In: Water Science and Technology, Vol. 48, No. 6, 2003, p. 9-16.

Research output: Contribution to journalArticle

Cervantes, FJ, Duong-Dac, T, Roest, K, Akkermans, ADL, Lettinga, G & Field, JA 2003, 'Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge', Water Science and Technology, vol. 48, no. 6, pp. 9-16.
Cervantes, F. J. ; Duong-Dac, T. ; Roest, K. ; Akkermans, A. D L ; Lettinga, G. ; Field, James A. / Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge. In: Water Science and Technology. 2003 ; Vol. 48, No. 6. pp. 9-16.
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