Enrichment of low-density symbiont DNA from minute insects

Corinne M. Stouthamer, Suzanne Kelly, Martha S Hunter

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Symbioses between bacteria and insects are often associated with changes in important biological traits that can significantly affect host fitness. To a large extent, studies of these interactions have been based on physiological changes or induced phenotypes in the host, and the genetic mechanisms by which symbionts interact with their hosts have only recently become better understood. Learning about symbionts has been challenging in part due to difficulties such as obtaining enough high quality genomic material for high throughput sequencing technology, especially for symbionts present in low titers, and in small or difficult to rear non-model hosts. Here we introduce a new method that substantially increases the yield of bacterial DNA in minute arthropod hosts, and requires less starting material relative to previous published methods.

Original languageEnglish (US)
Pages (from-to)16-19
Number of pages4
JournalJournal of Microbiological Methods
Volume151
DOIs
StatePublished - Aug 1 2018

Keywords

  • Cardinium
  • Low titer infections
  • Microbial DNA enrichment protocol
  • Parasitic wasps
  • Parasitoid
  • Symbiont
  • Wolbachia

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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