Environmental arsenic exposure and microbiota in induced sputum

Allison G. White, George S Watts, Zhenqiang Lu, Maria M. Meza-Montenegro, Eric A Lutz, Philip I Harber, Jefferey L Burgess

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Arsenic exposure from drinking water is associated with adverse respiratory outcomes, but it is unknown whether arsenic affects pulmonary microbiota. This exploratory study assessed the effect of exposure to arsenic in drinking water on bacterial diversity in the respiratory tract of non-smokers. Induced sputum was collected from 10 subjects with moderate mean household water arsenic concentration (21.1 ± 6.4 ppb) and 10 subjects with low household water arsenic (2.4 ± 0.8 ppb). To assess microbiota in sputum, the V6 hypervariable region amplicons of bacterial 16s rRNA genes were sequenced using the Ion Torrent Personal Genome Machine. Microbial community differences between arsenic exposure groups were evaluated using QIIME and Metastats. A total of 3,920,441 sequence reads, ranging from 37,935 to 508,787 per sample for 316 chips after QIIME quality filtering, were taxonomically classified into 142 individual genera and five phyla. Firmicutes (22%), Proteobacteria (17%) and Bacteriodetes (12%) were the main phyla in all samples, with Neisseriaceae (15%), Prevotellaceae (12%) and Veillonellacea (7%) being most common at the genus level. Some genera, including Gemella, Lactobacillales, Streptococcus, Neisseria and Pasteurellaceae were elevated in the moderate arsenic exposure group, while Rothia, Prevotella, Prevotellaceae Fusobacterium and Neisseriaceae were decreased, although none of these differences was statistically significant. Future studies with more participants and a greater range of arsenic exposure are needed to further elucidate the effects of drinking water arsenic consumption on respiratory microbiota.

Original languageEnglish (US)
Pages (from-to)2299-2313
Number of pages15
JournalInternational Journal of Environmental Research and Public Health
Volume11
Issue number2
DOIs
StatePublished - Feb 21 2014

Fingerprint

Microbiota
Environmental Exposure
Arsenic
Sputum
Neisseriaceae
Drinking Water
Lactobacillales
Gemella
Pasteurellaceae
Fusobacterium
Prevotella
Neisseria
Proteobacteria
Water
Streptococcus
rRNA Genes
Respiratory System
Drinking
Genome
Ions

Keywords

  • Arsenic
  • Microbiota
  • Sputum

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Environmental arsenic exposure and microbiota in induced sputum. / White, Allison G.; Watts, George S; Lu, Zhenqiang; Meza-Montenegro, Maria M.; Lutz, Eric A; Harber, Philip I; Burgess, Jefferey L.

In: International Journal of Environmental Research and Public Health, Vol. 11, No. 2, 21.02.2014, p. 2299-2313.

Research output: Contribution to journalArticle

@article{a5597890566a4eca894a54a99b6dd8c0,
title = "Environmental arsenic exposure and microbiota in induced sputum",
abstract = "Arsenic exposure from drinking water is associated with adverse respiratory outcomes, but it is unknown whether arsenic affects pulmonary microbiota. This exploratory study assessed the effect of exposure to arsenic in drinking water on bacterial diversity in the respiratory tract of non-smokers. Induced sputum was collected from 10 subjects with moderate mean household water arsenic concentration (21.1 ± 6.4 ppb) and 10 subjects with low household water arsenic (2.4 ± 0.8 ppb). To assess microbiota in sputum, the V6 hypervariable region amplicons of bacterial 16s rRNA genes were sequenced using the Ion Torrent Personal Genome Machine. Microbial community differences between arsenic exposure groups were evaluated using QIIME and Metastats. A total of 3,920,441 sequence reads, ranging from 37,935 to 508,787 per sample for 316 chips after QIIME quality filtering, were taxonomically classified into 142 individual genera and five phyla. Firmicutes (22{\%}), Proteobacteria (17{\%}) and Bacteriodetes (12{\%}) were the main phyla in all samples, with Neisseriaceae (15{\%}), Prevotellaceae (12{\%}) and Veillonellacea (7{\%}) being most common at the genus level. Some genera, including Gemella, Lactobacillales, Streptococcus, Neisseria and Pasteurellaceae were elevated in the moderate arsenic exposure group, while Rothia, Prevotella, Prevotellaceae Fusobacterium and Neisseriaceae were decreased, although none of these differences was statistically significant. Future studies with more participants and a greater range of arsenic exposure are needed to further elucidate the effects of drinking water arsenic consumption on respiratory microbiota.",
keywords = "Arsenic, Microbiota, Sputum",
author = "White, {Allison G.} and Watts, {George S} and Zhenqiang Lu and Meza-Montenegro, {Maria M.} and Lutz, {Eric A} and Harber, {Philip I} and Burgess, {Jefferey L}",
year = "2014",
month = "2",
day = "21",
doi = "10.3390/ijerph110202299",
language = "English (US)",
volume = "11",
pages = "2299--2313",
journal = "International Journal of Environmental Research and Public Health",
issn = "1661-7827",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

TY - JOUR

T1 - Environmental arsenic exposure and microbiota in induced sputum

AU - White, Allison G.

AU - Watts, George S

AU - Lu, Zhenqiang

AU - Meza-Montenegro, Maria M.

AU - Lutz, Eric A

AU - Harber, Philip I

AU - Burgess, Jefferey L

PY - 2014/2/21

Y1 - 2014/2/21

N2 - Arsenic exposure from drinking water is associated with adverse respiratory outcomes, but it is unknown whether arsenic affects pulmonary microbiota. This exploratory study assessed the effect of exposure to arsenic in drinking water on bacterial diversity in the respiratory tract of non-smokers. Induced sputum was collected from 10 subjects with moderate mean household water arsenic concentration (21.1 ± 6.4 ppb) and 10 subjects with low household water arsenic (2.4 ± 0.8 ppb). To assess microbiota in sputum, the V6 hypervariable region amplicons of bacterial 16s rRNA genes were sequenced using the Ion Torrent Personal Genome Machine. Microbial community differences between arsenic exposure groups were evaluated using QIIME and Metastats. A total of 3,920,441 sequence reads, ranging from 37,935 to 508,787 per sample for 316 chips after QIIME quality filtering, were taxonomically classified into 142 individual genera and five phyla. Firmicutes (22%), Proteobacteria (17%) and Bacteriodetes (12%) were the main phyla in all samples, with Neisseriaceae (15%), Prevotellaceae (12%) and Veillonellacea (7%) being most common at the genus level. Some genera, including Gemella, Lactobacillales, Streptococcus, Neisseria and Pasteurellaceae were elevated in the moderate arsenic exposure group, while Rothia, Prevotella, Prevotellaceae Fusobacterium and Neisseriaceae were decreased, although none of these differences was statistically significant. Future studies with more participants and a greater range of arsenic exposure are needed to further elucidate the effects of drinking water arsenic consumption on respiratory microbiota.

AB - Arsenic exposure from drinking water is associated with adverse respiratory outcomes, but it is unknown whether arsenic affects pulmonary microbiota. This exploratory study assessed the effect of exposure to arsenic in drinking water on bacterial diversity in the respiratory tract of non-smokers. Induced sputum was collected from 10 subjects with moderate mean household water arsenic concentration (21.1 ± 6.4 ppb) and 10 subjects with low household water arsenic (2.4 ± 0.8 ppb). To assess microbiota in sputum, the V6 hypervariable region amplicons of bacterial 16s rRNA genes were sequenced using the Ion Torrent Personal Genome Machine. Microbial community differences between arsenic exposure groups were evaluated using QIIME and Metastats. A total of 3,920,441 sequence reads, ranging from 37,935 to 508,787 per sample for 316 chips after QIIME quality filtering, were taxonomically classified into 142 individual genera and five phyla. Firmicutes (22%), Proteobacteria (17%) and Bacteriodetes (12%) were the main phyla in all samples, with Neisseriaceae (15%), Prevotellaceae (12%) and Veillonellacea (7%) being most common at the genus level. Some genera, including Gemella, Lactobacillales, Streptococcus, Neisseria and Pasteurellaceae were elevated in the moderate arsenic exposure group, while Rothia, Prevotella, Prevotellaceae Fusobacterium and Neisseriaceae were decreased, although none of these differences was statistically significant. Future studies with more participants and a greater range of arsenic exposure are needed to further elucidate the effects of drinking water arsenic consumption on respiratory microbiota.

KW - Arsenic

KW - Microbiota

KW - Sputum

UR - http://www.scopus.com/inward/record.url?scp=84896871488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896871488&partnerID=8YFLogxK

U2 - 10.3390/ijerph110202299

DO - 10.3390/ijerph110202299

M3 - Article

C2 - 24566055

AN - SCOPUS:84896871488

VL - 11

SP - 2299

EP - 2313

JO - International Journal of Environmental Research and Public Health

JF - International Journal of Environmental Research and Public Health

SN - 1661-7827

IS - 2

ER -