Environmental design considerations for somatic embryogenesis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In addition to the biomolecular, physiological, and biochemical aspects of somatic embryogenesis, careful design of environmental conditions is necessary to ensure the successful induction and development of somatic embryos for different plant species. A dissolved oxygen concentration, for instance, below 10% generally inhibits the differentiation of somatic embryos, while the same is promoted at 40, 80, or 100%, depending on the plant species. Certain plant species also exhibit inhibition of somatic embryo differentiation at high dissolved oxygen concentration, such as at 80%. Cell density influences somatic embryogenesis by changing the concentrations of conditioning factors released by plant cells and embryos into the culture medium. High initial cell density, in general, results in inhibition of somatic embryo differentiation on account of inhibitory compounds released by cells into the culture medium. Partial medium replacement has been employed to rectify this situation. In terms of the general influence of light, red light promotes and blue light inhibits the induction of somatic embryos. Blue light, however, generally promotes the development of somatic embryos.

Original languageEnglish (US)
Pages (from-to)25-34
Number of pages10
JournalPlant Cell Monographs
Volume2
DOIs
StatePublished - Nov 30 2005

Fingerprint

design for environment
somatic embryos
somatic embryogenesis
Embryonic Development
Embryonic Structures
Light
Culture Media
Seeds
Cell Count
blue light
Oxygen
dissolved oxygen
Plant Cells
culture media
Cell Culture Techniques
embryo culture
cells
red light
cell culture
environmental factors

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Environmental design considerations for somatic embryogenesis. / Hoshino, Takanori -; Cuello, Joel L.

In: Plant Cell Monographs, Vol. 2, 30.11.2005, p. 25-34.

Research output: Contribution to journalArticle

@article{9652fb3582ca4743af79a65c1dc57f14,
title = "Environmental design considerations for somatic embryogenesis",
abstract = "In addition to the biomolecular, physiological, and biochemical aspects of somatic embryogenesis, careful design of environmental conditions is necessary to ensure the successful induction and development of somatic embryos for different plant species. A dissolved oxygen concentration, for instance, below 10{\%} generally inhibits the differentiation of somatic embryos, while the same is promoted at 40, 80, or 100{\%}, depending on the plant species. Certain plant species also exhibit inhibition of somatic embryo differentiation at high dissolved oxygen concentration, such as at 80{\%}. Cell density influences somatic embryogenesis by changing the concentrations of conditioning factors released by plant cells and embryos into the culture medium. High initial cell density, in general, results in inhibition of somatic embryo differentiation on account of inhibitory compounds released by cells into the culture medium. Partial medium replacement has been employed to rectify this situation. In terms of the general influence of light, red light promotes and blue light inhibits the induction of somatic embryos. Blue light, however, generally promotes the development of somatic embryos.",
author = "Hoshino, {Takanori -} and Cuello, {Joel L}",
year = "2005",
month = "11",
day = "30",
doi = "10.1007/7089_035",
language = "English (US)",
volume = "2",
pages = "25--34",
journal = "Plant Cell Monographs",
issn = "1861-1370",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - Environmental design considerations for somatic embryogenesis

AU - Hoshino, Takanori -

AU - Cuello, Joel L

PY - 2005/11/30

Y1 - 2005/11/30

N2 - In addition to the biomolecular, physiological, and biochemical aspects of somatic embryogenesis, careful design of environmental conditions is necessary to ensure the successful induction and development of somatic embryos for different plant species. A dissolved oxygen concentration, for instance, below 10% generally inhibits the differentiation of somatic embryos, while the same is promoted at 40, 80, or 100%, depending on the plant species. Certain plant species also exhibit inhibition of somatic embryo differentiation at high dissolved oxygen concentration, such as at 80%. Cell density influences somatic embryogenesis by changing the concentrations of conditioning factors released by plant cells and embryos into the culture medium. High initial cell density, in general, results in inhibition of somatic embryo differentiation on account of inhibitory compounds released by cells into the culture medium. Partial medium replacement has been employed to rectify this situation. In terms of the general influence of light, red light promotes and blue light inhibits the induction of somatic embryos. Blue light, however, generally promotes the development of somatic embryos.

AB - In addition to the biomolecular, physiological, and biochemical aspects of somatic embryogenesis, careful design of environmental conditions is necessary to ensure the successful induction and development of somatic embryos for different plant species. A dissolved oxygen concentration, for instance, below 10% generally inhibits the differentiation of somatic embryos, while the same is promoted at 40, 80, or 100%, depending on the plant species. Certain plant species also exhibit inhibition of somatic embryo differentiation at high dissolved oxygen concentration, such as at 80%. Cell density influences somatic embryogenesis by changing the concentrations of conditioning factors released by plant cells and embryos into the culture medium. High initial cell density, in general, results in inhibition of somatic embryo differentiation on account of inhibitory compounds released by cells into the culture medium. Partial medium replacement has been employed to rectify this situation. In terms of the general influence of light, red light promotes and blue light inhibits the induction of somatic embryos. Blue light, however, generally promotes the development of somatic embryos.

UR - http://www.scopus.com/inward/record.url?scp=43349093605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43349093605&partnerID=8YFLogxK

U2 - 10.1007/7089_035

DO - 10.1007/7089_035

M3 - Article

AN - SCOPUS:43349093605

VL - 2

SP - 25

EP - 34

JO - Plant Cell Monographs

JF - Plant Cell Monographs

SN - 1861-1370

ER -