Enzyme-linked immunosorbent assay for antigens of Coccidioides immitis

Human sera interference corrected by acidification-heat extraction

Elizabeth E. Wack, Kris O. Dugger, John N Galgiani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe the distortion caused by sera from patients with coccidioidomycosis in an enzyme-linked immunosorbent assay (ELISA) for coccidioidal antigen. In experimental mixtures without exogenous antigen, immune sera produced an antigen-like effect. In contrast, in mixtures with a relatively large concentration of exogenous antigen, immune sera obscured antigen activity. In both circumstances, preparing specimens with an acidification-heat extraction procedure before assay rectified serum interference, and only in specimens with no exogenous antigen was the correction incomplete. Other evidence, including direct measurement of anticoccidioidal antibody after extraction, suggested that the residual antigen-like activity was not from persistent antibody. Incorporating the extraction procedure into the ELISA of clinical specimens, we found antigen activity in nine of 15 specimens. We conclude that acidificationheat extraction of sera improves detection of coccidioidal antigens and that coccidioidal antigens are present in some patients with coccidioidal infection.

Original languageEnglish (US)
Pages (from-to)548-559
Number of pages12
JournalThe Journal of Laboratory and Clinical Medicine
Volume111
Issue number5
StatePublished - 1988

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Coccidioides
Immunosorbents
Acidification
Assays
Hot Temperature
Enzyme-Linked Immunosorbent Assay
Antigens
Enzymes
Serum
Immune Sera
Coccidioidomycosis
Antibodies

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

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title = "Enzyme-linked immunosorbent assay for antigens of Coccidioides immitis: Human sera interference corrected by acidification-heat extraction",
abstract = "We describe the distortion caused by sera from patients with coccidioidomycosis in an enzyme-linked immunosorbent assay (ELISA) for coccidioidal antigen. In experimental mixtures without exogenous antigen, immune sera produced an antigen-like effect. In contrast, in mixtures with a relatively large concentration of exogenous antigen, immune sera obscured antigen activity. In both circumstances, preparing specimens with an acidification-heat extraction procedure before assay rectified serum interference, and only in specimens with no exogenous antigen was the correction incomplete. Other evidence, including direct measurement of anticoccidioidal antibody after extraction, suggested that the residual antigen-like activity was not from persistent antibody. Incorporating the extraction procedure into the ELISA of clinical specimens, we found antigen activity in nine of 15 specimens. We conclude that acidificationheat extraction of sera improves detection of coccidioidal antigens and that coccidioidal antigens are present in some patients with coccidioidal infection.",
author = "Wack, {Elizabeth E.} and Dugger, {Kris O.} and Galgiani, {John N}",
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T1 - Enzyme-linked immunosorbent assay for antigens of Coccidioides immitis

T2 - Human sera interference corrected by acidification-heat extraction

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AU - Dugger, Kris O.

AU - Galgiani, John N

PY - 1988

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N2 - We describe the distortion caused by sera from patients with coccidioidomycosis in an enzyme-linked immunosorbent assay (ELISA) for coccidioidal antigen. In experimental mixtures without exogenous antigen, immune sera produced an antigen-like effect. In contrast, in mixtures with a relatively large concentration of exogenous antigen, immune sera obscured antigen activity. In both circumstances, preparing specimens with an acidification-heat extraction procedure before assay rectified serum interference, and only in specimens with no exogenous antigen was the correction incomplete. Other evidence, including direct measurement of anticoccidioidal antibody after extraction, suggested that the residual antigen-like activity was not from persistent antibody. Incorporating the extraction procedure into the ELISA of clinical specimens, we found antigen activity in nine of 15 specimens. We conclude that acidificationheat extraction of sera improves detection of coccidioidal antigens and that coccidioidal antigens are present in some patients with coccidioidal infection.

AB - We describe the distortion caused by sera from patients with coccidioidomycosis in an enzyme-linked immunosorbent assay (ELISA) for coccidioidal antigen. In experimental mixtures without exogenous antigen, immune sera produced an antigen-like effect. In contrast, in mixtures with a relatively large concentration of exogenous antigen, immune sera obscured antigen activity. In both circumstances, preparing specimens with an acidification-heat extraction procedure before assay rectified serum interference, and only in specimens with no exogenous antigen was the correction incomplete. Other evidence, including direct measurement of anticoccidioidal antibody after extraction, suggested that the residual antigen-like activity was not from persistent antibody. Incorporating the extraction procedure into the ELISA of clinical specimens, we found antigen activity in nine of 15 specimens. We conclude that acidificationheat extraction of sera improves detection of coccidioidal antigens and that coccidioidal antigens are present in some patients with coccidioidal infection.

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