TY - JOUR
T1 - Enzymological studies of melanotropins
AU - Castrucci, Ana Maria de L.
AU - Hadley, Mac E.
AU - Sawyer, Tomi K.
AU - Hruby, Victor J.
PY - 1984
Y1 - 1984
N2 - 1. 1. The relative stability of natural melanotropins and related synthetic analogues to serum and purified proteolytic enzymes was studied. 2. 2. Both α- and β-MSH were rapidly inactivated by frog serum, but much more slowly by rat serum. β-MSH was more stable than α-MSH to serum inactivation. 3. 3. Both α- and β-MSH were rapidly inactivated by α-chymotrypsin and trypsin. 4. 4. The synthetic analogues, [Nle4, d-Phe7]-α-MSH and and [Cys4,Cys10]-α-MSH, were totally resistant to inactivation by frog and rat serum enzymes. 5. 5. [Nle4, d-Phe7]-α-MSH was resistant to inactivation by α-chymotrypsin and trypsin, whereas [Cys4,Cys10]-α-MSH, was partially resistant to these enzymes under similar conditions. 6. 6. Melanotropin analogues resistant to inactivation by serum enzymes may prove useful in a variety of physiological studies wherein natural melanotropins would be rapidly inactivated.
AB - 1. 1. The relative stability of natural melanotropins and related synthetic analogues to serum and purified proteolytic enzymes was studied. 2. 2. Both α- and β-MSH were rapidly inactivated by frog serum, but much more slowly by rat serum. β-MSH was more stable than α-MSH to serum inactivation. 3. 3. Both α- and β-MSH were rapidly inactivated by α-chymotrypsin and trypsin. 4. 4. The synthetic analogues, [Nle4, d-Phe7]-α-MSH and and [Cys4,Cys10]-α-MSH, were totally resistant to inactivation by frog and rat serum enzymes. 5. 5. [Nle4, d-Phe7]-α-MSH was resistant to inactivation by α-chymotrypsin and trypsin, whereas [Cys4,Cys10]-α-MSH, was partially resistant to these enzymes under similar conditions. 6. 6. Melanotropin analogues resistant to inactivation by serum enzymes may prove useful in a variety of physiological studies wherein natural melanotropins would be rapidly inactivated.
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U2 - 10.1016/0305-0491(84)90090-7
DO - 10.1016/0305-0491(84)90090-7
M3 - Article
C2 - 6332706
AN - SCOPUS:0021227377
VL - 78
SP - 519
EP - 524
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 3
ER -