Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury

Radhakrishna Rao, Frank Porreca

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 ± 4.4% decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalEuropean Journal of Pharmacology
Volume303
Issue number3
DOIs
StatePublished - May 15 1996

Fingerprint

Octoxynol
Epidermal Growth Factor
Mucous Membrane
Wounds and Injuries
Buffers

Keywords

  • EGF (epidermal growth factor)
  • epithelium
  • mucosal protection
  • receptor
  • tissue resistance

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury. / Rao, Radhakrishna; Porreca, Frank.

In: European Journal of Pharmacology, Vol. 303, No. 3, 15.05.1996, p. 209-212.

Research output: Contribution to journalArticle

@article{c442490c09604650a56a6475cca34b62,
title = "Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury",
abstract = "Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3{\%} Triton X-100 for 10 min resulted in a 45.3 ± 4.4{\%} decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5{\%}. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.",
keywords = "EGF (epidermal growth factor), epithelium, mucosal protection, receptor, tissue resistance",
author = "Radhakrishna Rao and Frank Porreca",
year = "1996",
month = "5",
day = "15",
doi = "10.1016/0014-2999(96)00186-0",
language = "English (US)",
volume = "303",
pages = "209--212",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury

AU - Rao, Radhakrishna

AU - Porreca, Frank

PY - 1996/5/15

Y1 - 1996/5/15

N2 - Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 ± 4.4% decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.

AB - Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 ± 4.4% decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.

KW - EGF (epidermal growth factor)

KW - epithelium

KW - mucosal protection

KW - receptor

KW - tissue resistance

UR - http://www.scopus.com/inward/record.url?scp=0029950783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029950783&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(96)00186-0

DO - 10.1016/0014-2999(96)00186-0

M3 - Article

VL - 303

SP - 209

EP - 212

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 3

ER -