Abstract
Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 ± 4.4% decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 209-212 |
| Number of pages | 4 |
| Journal | European Journal of Pharmacology |
| Volume | 303 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 15 1996 |
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Keywords
- EGF (epidermal growth factor)
- epithelium
- mucosal protection
- receptor
- tissue resistance
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury. / Rao, Radhakrishna; Porreca, Frank.
In: European Journal of Pharmacology, Vol. 303, No. 3, 15.05.1996, p. 209-212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epidermal growth factor protects mouse ileal mucosa from Triton X-100-induced injury
AU - Rao, Radhakrishna
AU - Porreca, Frank
PY - 1996/5/15
Y1 - 1996/5/15
N2 - Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 ± 4.4% decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.
AB - Luminal exposure of mouse ileal mucosal sheets in vitro to 0.3% Triton X-100 for 10 min resulted in a 45.3 ± 4.4% decrease in tissue resistance (R(t)). Luminal pretreatment of tissue with epidermal growth factor (EGF, 30 nM) reduced the Triton X-100-induced decrease in R(t) to 10.2 ± 3.5%. This effect of luminal EGF was concentration-related with an A50 value of 7.4 nM. EGF applied to the basal buffer also produced a similar protective effect, but it was 5.3-fold less potent than luminal EGF. Morphological analysis indicated the formation of denudations at villi tips by Triton X-100 which was prevented by EGF pretreatment. These results suggest that EGF may play a role in the protection of ileal mucosal integrity.
KW - EGF (epidermal growth factor)
KW - epithelium
KW - mucosal protection
KW - receptor
KW - tissue resistance
UR - http://www.scopus.com/inward/record.url?scp=0029950783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029950783&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(96)00186-0
DO - 10.1016/0014-2999(96)00186-0
M3 - Article
VL - 303
SP - 209
EP - 212
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 3
ER -