Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis

Andrew A. Maynard, Katerina Dvorak, Ludmila Khailova, Holly Dobrenen, Kelly M. Arganbright, Melissa D Halpern, Ashish R. Kurundkar, Akhil Maheshwari, Bohuslav Dvorak

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Necrotizing enterocolitis (NEC) is a devastating intestinal disease of premature infants. Epidermal growth factor (EGF) is one of the most promising candidates in NEC prophylaxis. Autophagy regulates cell homeostasis, but uncontrolled activation of autophagy may lead to cellular injury. The aim was to evaluate the effects of EGF on intestinal autophagy in epithelial cells and in the rat NEC model and measure autophagy in NEC patients. Intestinal epithelial cells (IEC-6) and the rat NEC model were used to study the effect of EGF on intestinal autophagy. Protein levels of Beclin 1 and LC3II were measured in the intestinal epithelium in both in vivo and in vitro models. Ultrastructural changes in intestinal epithelium were studied by electron microscopy. Expression of Beclin 1, LC3II, and p62 protein was evaluated in biopsies from NEC patients. Autophagy was induced in IEC-6 cells and inhibited by adding EGF into the culture. In the rat NEC model, EGF treatment of NEC reduced expression of Beclin 1 and LC3II in ileal epithelium. Morphologically, typical signs of autophagy were observed in the epithelium of the NEC group, but not in the EGF group. A strong signal for Beclin 1 and LC3II was detected in the intestine from patients with NEC. Autophagy is activated in the intestinal epithelium of NEC patients and in the ileum of NEC rats. Supplementation of EGF blocks intestinal autophagy in both in vivo and in vitro conditions. Results from this study indicate that EGFmediated protection against NEC injury is associated with regulation of intestinal autophagy.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume299
Issue number3
DOIs
StatePublished - Sep 2010

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Necrotizing Enterocolitis
Autophagy
Intestinal Mucosa
Epidermal Growth Factor
Epithelium
Epithelial Cells
Intestinal Diseases
Wounds and Injuries
Ileum
Premature Infants
Intestines
Electron Microscopy

Keywords

  • Enteral nutrition
  • Epithelial homeostasis
  • Premature
  • Small intestine

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology (medical)
  • Physiology
  • Hepatology
  • Medicine(all)

Cite this

Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis. / Maynard, Andrew A.; Dvorak, Katerina; Khailova, Ludmila; Dobrenen, Holly; Arganbright, Kelly M.; Halpern, Melissa D; Kurundkar, Ashish R.; Maheshwari, Akhil; Dvorak, Bohuslav.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 299, No. 3, 09.2010.

Research output: Contribution to journalArticle

Maynard, Andrew A. ; Dvorak, Katerina ; Khailova, Ludmila ; Dobrenen, Holly ; Arganbright, Kelly M. ; Halpern, Melissa D ; Kurundkar, Ashish R. ; Maheshwari, Akhil ; Dvorak, Bohuslav. / Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2010 ; Vol. 299, No. 3.
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