Epigenetic silencing of lncRNA MORT in 16 TCGA cancer types

Lukas Vrba, Bernard W Futscher

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have previously described a hominid-specific long non-coding RNA, MORT (also known as ZNF667-AS1, Gene ID: 100128252), which is expressed in all normal cell types, but epigenetically silenced during cancer-associated immortalization of human mammary epithelial cells. Initial analysis of The Cancer Genome Atlas (TCGA) showed that 15 of 17 cancer types, which represent the 10 most common cancers in women and men, display DNA methylation associated MORT silencing in a large fraction of their tumors. In this study we analyzed MORT expression and DNA methylation state in the remaining 16 TCGA cancer types not previously reported. Seven of the 16 cancer types showed DNA methylation linked MORT silencing in a large fraction of their tumors. These are carcinomas (cervical cancer, and cancers of esophagus, stomach, and bile duct), and the non-epithelial tumors mesothelioma, sarcoma, and uterine carcinosarcoma. Together with the findings from our previous report, MORT expression is silenced by aberrant DNA methylation in 22 of 33 of TCGA cancer types. These 22 cancers include most carcinoma types, blood derived cancers and sarcomas. In conclusion, results suggest that the MORT gene is one of the most common epigenetic aberrations seen in human cancer. Coupled with the timing of MORT gene silencing during in vitro epithelial cell immortalization and its occurrence early in the temporal arc of human carcinogenesis, this provides strong circumstantial evidence for a tumor suppressor role for MORT.

Original languageEnglish (US)
Article number211
JournalF1000Research
Volume7
DOIs
StatePublished - Jan 1 2018

Fingerprint

Long Noncoding RNA
Atlases
Epigenomics
Genes
Genome
Tumors
Neoplasms
DNA Methylation
Aberrations
Ducts
Blood
Display devices
Sarcoma
Epithelial Cells
Carcinoma
Carcinosarcoma

Keywords

  • DNA Methylation
  • Epigenetics
  • Gene Silencing
  • LincRNA
  • LncRNA
  • MORT
  • NcRNA
  • ZNF667-AS1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Epigenetic silencing of lncRNA MORT in 16 TCGA cancer types. / Vrba, Lukas; Futscher, Bernard W.

In: F1000Research, Vol. 7, 211, 01.01.2018.

Research output: Contribution to journalArticle

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