Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides

A. Suchankova, V. Krchnak, Josef Vagner, E. Hamsikova, M. Krcmar, L. Ritterova, V. Vonka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Eight overlapping icosapeptides covering the entire sequence of the E4 protein of human papillomavirus type 16 (HPV-16), were prepared and tested for their reactivity with human sera in IgG-specific ELISA. The strongest reactivity of sera from HPV-16 DNA-positive invasive cervical carcinoma (INCA) patients was detected with the peptide denoted 16/E4-6, covering amino acids 51 to 70. Subsequently nearly 200 sera were tested for the presence of the 16/E4-6-specific antibody. Reactivity was more frequent in cervical intraepithelial neoplasia patients and INCA patients than in matched control subjects. Sera from INCA patients were also tested for antibody reactive with peptide 16/E7-2 covering the major type-specific reactive region of the HPV- 16/E7 protein. Only four of 13 sera possessing the 16/E4-6-specific antibody were reactive with the 16/E7-2 peptide.

Original languageEnglish (US)
Pages (from-to)429-432
Number of pages4
JournalJournal of General Virology
Volume73
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Epitope Mapping
Human papillomavirus 16
Peptides
Serum
Proteins
Carcinoma
Antibodies
Cervical Intraepithelial Neoplasia
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Amino Acids
DNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Suchankova, A., Krchnak, V., Vagner, J., Hamsikova, E., Krcmar, M., Ritterova, L., & Vonka, V. (1992). Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides. Journal of General Virology, 73(2), 429-432.

Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides. / Suchankova, A.; Krchnak, V.; Vagner, Josef; Hamsikova, E.; Krcmar, M.; Ritterova, L.; Vonka, V.

In: Journal of General Virology, Vol. 73, No. 2, 1992, p. 429-432.

Research output: Contribution to journalArticle

Suchankova, A, Krchnak, V, Vagner, J, Hamsikova, E, Krcmar, M, Ritterova, L & Vonka, V 1992, 'Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides', Journal of General Virology, vol. 73, no. 2, pp. 429-432.
Suchankova A, Krchnak V, Vagner J, Hamsikova E, Krcmar M, Ritterova L et al. Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides. Journal of General Virology. 1992;73(2):429-432.
Suchankova, A. ; Krchnak, V. ; Vagner, Josef ; Hamsikova, E. ; Krcmar, M. ; Ritterova, L. ; Vonka, V. / Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides. In: Journal of General Virology. 1992 ; Vol. 73, No. 2. pp. 429-432.
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