Eriodictyol-7-O-glucoside, a novel Nrf2 activator, confers protection against cisplatin-induced toxicity

Qingwen Hu, Donna Zhang, Limei Wang, Hongxiang Lou, Dongmei Ren

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Eriodictyol-7- O-glucoside, a flavonoid isolated from Dracocephalum rupestre, is among the most potent free radical scavenger. In the present study, we identified eriodictyol-7- O-glucoside as a novel nuclear factor E2-related factor 2 (Nrf2) activator using a high-throughput cellular screening method. This compound activated Nrf2 signaling pathway and was able to stabilize Nrf2 by delaying Nrf2 degradation, resulting in accumulation of Nrf2 protein and activation of the Nrf2-dependent protective response. Recent studies have suggested that activation of Nrf2 pathway would confer protection against cisplatin-induced toxicity. The protective role of eriodictyol-7- O-glucoside in cisplatin-induced toxicity was investigated in a human renal mesangial cell line, HRMC. Cotreatment of HRMC cells with eriodictyol-7- O-glucoside significantly improved cell survival under cisplatin exposure. These findings demonstrated the feasibility of using natural compounds targeting Nrf2 as a therapeutic approach to subvert the side effects of cisplatin in normal cells.

Original languageEnglish (US)
Pages (from-to)1927-1932
Number of pages6
JournalFood and Chemical Toxicology
Volume50
Issue number6
DOIs
StatePublished - Jun 2012

Fingerprint

NF-E2-Related Factor 2
cisplatin
Glucosides
Cisplatin
glucosides
Toxicity
toxicity
Dracocephalum
free radical scavengers
kidney cells
cell viability
flavonoids
Chemical activation
adverse effects
cell lines
cells
High-Throughput Screening Assays
screening
therapeutics
Free Radical Scavengers

Keywords

  • Activate
  • Cisplatin
  • Eriodictyol-7-O-glucoside
  • Nrf2
  • Toxicity

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Eriodictyol-7-O-glucoside, a novel Nrf2 activator, confers protection against cisplatin-induced toxicity. / Hu, Qingwen; Zhang, Donna; Wang, Limei; Lou, Hongxiang; Ren, Dongmei.

In: Food and Chemical Toxicology, Vol. 50, No. 6, 06.2012, p. 1927-1932.

Research output: Contribution to journalArticle

Hu, Qingwen ; Zhang, Donna ; Wang, Limei ; Lou, Hongxiang ; Ren, Dongmei. / Eriodictyol-7-O-glucoside, a novel Nrf2 activator, confers protection against cisplatin-induced toxicity. In: Food and Chemical Toxicology. 2012 ; Vol. 50, No. 6. pp. 1927-1932.
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