Error analysis of ratiometric imaging of extracellular pH in a window chamber model

Yuxiang Lin, Tzu Yu Wu, Arthur F Gmitro

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ratiometric fluorescence-imaging technique is commonly used to measure extracellular pH in tumors and surrounding tissue within a dorsal skin-fold window chamber. Using a pH-sensitive fluorophore such as carboxy SNARF-1 one can measure pH distributions with high precision. However, it is often observed that the measured pH is lower than expected, with a bias that varies from one image to another. A comprehensive analysis of possible error sources is presented. These error sources include photon noise, estimator bias, instrument errors, temperature, and calibration errors from biological factors.

Original languageEnglish (US)
Article number046004-1
JournalJournal of Biomedical Optics
Volume17
Issue number4
DOIs
StatePublished - Apr 2012

Fingerprint

error analysis
Error analysis
chambers
Imaging techniques
Instrument errors
instrument errors
Fluorophores
Biological Factors
Tumors
Skin
Photons
Fluorescence
estimators
imaging techniques
Calibration
Tissue
tumors
fluorescence
photons
Temperature

Keywords

  • Fluorescence
  • Image quality
  • Imaging
  • Microscopy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Error analysis of ratiometric imaging of extracellular pH in a window chamber model. / Lin, Yuxiang; Wu, Tzu Yu; Gmitro, Arthur F.

In: Journal of Biomedical Optics, Vol. 17, No. 4, 046004-1, 04.2012.

Research output: Contribution to journalArticle

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