Errors in confocal fluorescence ratiometric imaging microscopy due to chromatic aberration

Yuxiang Lin, Arthur F. Gmitro

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Confocal fluorescence ratiometric imaging is an optical technique used to measure a variety of important biological parameters. A small amount of chromatic aberration in the microscope system can introduce a variation in the signal ratio dependent on the fluorophore concentration gradient along the optical axis and lead to bias in the measurement. We present a theoretical model of this effect. Experimental results and simulations clearly demonstrate that this error can be significant and should not be ignored.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalApplied optics
Volume50
Issue number1
DOIs
StatePublished - Jan 1 2011

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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