Abstract
A cell-line (UACC 2561), was established from a biopsy specimen of a lung carcinosarcoma. The patient had Stage III non-small cell lung cancer. Characterization of this cell line revealed highly anueploid cells containing multiple clonal chromosome alterations with growth in nude mice within 3 weeks following subcutaneous injection. The cell line UACC 2561 stained positive for vimentin and displayed resistance to a variety of chemotherapeutic agents. Polymerase chain reaction followed by single-strand conformational polymorphism analysis revealed the presence of a K-ras 12 codon mutation. This mutation was confirmed by mutation specific PCR analysis for mutant K-ras alleles and direct DNA sequencing to be a GGT to GTT at codon 12 of the K-ras gene. Examination by PCR-SSCP for p53 mutations did not reveal any point mutations in exon 5-8 of the p53 gene. This relatively rare cell line may represent a useful model to investigate human lung sarcomas.
Original language | English (US) |
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Pages (from-to) | 145-152 |
Number of pages | 8 |
Journal | Cancer Letters |
Volume | 82 |
Issue number | 2 |
DOIs | |
State | Published - Jul 29 1994 |
Keywords
- Carcinoma
- Cell line
- Clonal chromosome alterations
- Lung
ASJC Scopus subject areas
- Oncology
- Cancer Research