Establishment of a new lung sarcoma cell line from a human lung carcinosarcoma

Albert Leibovitz, Julie Wymer, Kathy Massey, Floyd H. Thompson, Mark A. Nelson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A cell-line (UACC 2561), was established from a biopsy specimen of a lung carcinosarcoma. The patient had Stage III non-small cell lung cancer. Characterization of this cell line revealed highly anueploid cells containing multiple clonal chromosome alterations with growth in nude mice within 3 weeks following subcutaneous injection. The cell line UACC 2561 stained positive for vimentin and displayed resistance to a variety of chemotherapeutic agents. Polymerase chain reaction followed by single-strand conformational polymorphism analysis revealed the presence of a K-ras 12 codon mutation. This mutation was confirmed by mutation specific PCR analysis for mutant K-ras alleles and direct DNA sequencing to be a GGT to GTT at codon 12 of the K-ras gene. Examination by PCR-SSCP for p53 mutations did not reveal any point mutations in exon 5-8 of the p53 gene. This relatively rare cell line may represent a useful model to investigate human lung sarcomas.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalCancer Letters
Volume82
Issue number2
DOIs
StatePublished - Jul 29 1994

Keywords

  • Carcinoma
  • Cell line
  • Clonal chromosome alterations
  • Lung

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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