Abstract
A cell-line (UACC 2561), was established from a biopsy specimen of a lung carcinosarcoma. The patient had Stage III non-small cell lung cancer. Characterization of this cell line revealed highly anueploid cells containing multiple clonal chromosome alterations with growth in nude mice within 3 weeks following subcutaneous injection. The cell line UACC 2561 stained positive for vimentin and displayed resistance to a variety of chemotherapeutic agents. Polymerase chain reaction followed by single-strand conformational polymorphism analysis revealed the presence of a K-ras 12 codon mutation. This mutation was confirmed by mutation specific PCR analysis for mutant K-ras alleles and direct DNA sequencing to be a GGT to GTT at codon 12 of the K-ras gene. Examination by PCR-SSCP for p53 mutations did not reveal any point mutations in exon 5-8 of the p53 gene. This relatively rare cell line may represent a useful model to investigate human lung sarcomas.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-152 |
| Number of pages | 8 |
| Journal | Cancer Letters |
| Volume | 82 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 29 1994 |
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Keywords
- Carcinoma
- Cell line
- Clonal chromosome alterations
- Lung
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Establishment of a new lung sarcoma cell line from a human lung carcinosarcoma. / Leibovitz, Albert; Wymer, Julie; Massey, Kathy; Thompson, Floyd H.; Nelson, Mark A.
In: Cancer Letters, Vol. 82, No. 2, 29.07.1994, p. 145-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment of a new lung sarcoma cell line from a human lung carcinosarcoma
AU - Leibovitz, Albert
AU - Wymer, Julie
AU - Massey, Kathy
AU - Thompson, Floyd H.
AU - Nelson, Mark A
PY - 1994/7/29
Y1 - 1994/7/29
N2 - A cell-line (UACC 2561), was established from a biopsy specimen of a lung carcinosarcoma. The patient had Stage III non-small cell lung cancer. Characterization of this cell line revealed highly anueploid cells containing multiple clonal chromosome alterations with growth in nude mice within 3 weeks following subcutaneous injection. The cell line UACC 2561 stained positive for vimentin and displayed resistance to a variety of chemotherapeutic agents. Polymerase chain reaction followed by single-strand conformational polymorphism analysis revealed the presence of a K-ras 12 codon mutation. This mutation was confirmed by mutation specific PCR analysis for mutant K-ras alleles and direct DNA sequencing to be a GGT to GTT at codon 12 of the K-ras gene. Examination by PCR-SSCP for p53 mutations did not reveal any point mutations in exon 5-8 of the p53 gene. This relatively rare cell line may represent a useful model to investigate human lung sarcomas.
AB - A cell-line (UACC 2561), was established from a biopsy specimen of a lung carcinosarcoma. The patient had Stage III non-small cell lung cancer. Characterization of this cell line revealed highly anueploid cells containing multiple clonal chromosome alterations with growth in nude mice within 3 weeks following subcutaneous injection. The cell line UACC 2561 stained positive for vimentin and displayed resistance to a variety of chemotherapeutic agents. Polymerase chain reaction followed by single-strand conformational polymorphism analysis revealed the presence of a K-ras 12 codon mutation. This mutation was confirmed by mutation specific PCR analysis for mutant K-ras alleles and direct DNA sequencing to be a GGT to GTT at codon 12 of the K-ras gene. Examination by PCR-SSCP for p53 mutations did not reveal any point mutations in exon 5-8 of the p53 gene. This relatively rare cell line may represent a useful model to investigate human lung sarcomas.
KW - Carcinoma
KW - Cell line
KW - Clonal chromosome alterations
KW - Lung
UR - http://www.scopus.com/inward/record.url?scp=0028142237&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028142237&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(94)90005-1
DO - 10.1016/0304-3835(94)90005-1
M3 - Article
C2 - 8050085
AN - SCOPUS:0028142237
VL - 82
SP - 145
EP - 152
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -