Establishment of a new lung sarcoma cell line from a human lung carcinosarcoma

Albert Leibovitz, Julie Wymer, Kathy Massey, Floyd H. Thompson, Mark A Nelson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A cell-line (UACC 2561), was established from a biopsy specimen of a lung carcinosarcoma. The patient had Stage III non-small cell lung cancer. Characterization of this cell line revealed highly anueploid cells containing multiple clonal chromosome alterations with growth in nude mice within 3 weeks following subcutaneous injection. The cell line UACC 2561 stained positive for vimentin and displayed resistance to a variety of chemotherapeutic agents. Polymerase chain reaction followed by single-strand conformational polymorphism analysis revealed the presence of a K-ras 12 codon mutation. This mutation was confirmed by mutation specific PCR analysis for mutant K-ras alleles and direct DNA sequencing to be a GGT to GTT at codon 12 of the K-ras gene. Examination by PCR-SSCP for p53 mutations did not reveal any point mutations in exon 5-8 of the p53 gene. This relatively rare cell line may represent a useful model to investigate human lung sarcomas.

Original languageEnglish (US)
Pages (from-to)145-152
Number of pages8
JournalCancer Letters
Volume82
Issue number2
DOIs
StatePublished - Jul 29 1994

Fingerprint

Carcinosarcoma
Sarcoma
Cell Line
Lung
Mutation
Codon
Polymerase Chain Reaction
Single-Stranded Conformational Polymorphism
ras Genes
p53 Genes
Vimentin
Subcutaneous Injections
DNA Sequence Analysis
Point Mutation
Nude Mice
Non-Small Cell Lung Carcinoma
Exons
Chromosomes
Alleles
Biopsy

Keywords

  • Carcinoma
  • Cell line
  • Clonal chromosome alterations
  • Lung

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Establishment of a new lung sarcoma cell line from a human lung carcinosarcoma. / Leibovitz, Albert; Wymer, Julie; Massey, Kathy; Thompson, Floyd H.; Nelson, Mark A.

In: Cancer Letters, Vol. 82, No. 2, 29.07.1994, p. 145-152.

Research output: Contribution to journalArticle

Leibovitz, Albert ; Wymer, Julie ; Massey, Kathy ; Thompson, Floyd H. ; Nelson, Mark A. / Establishment of a new lung sarcoma cell line from a human lung carcinosarcoma. In: Cancer Letters. 1994 ; Vol. 82, No. 2. pp. 145-152.
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