Establishment of hamster blastocyst-derived embryonic stem (ES) cells

Thomas Doetschman, Phillip Williams, Nobuyo Maeda

Research output: Contribution to journalArticle

159 Scopus citations

Abstract

The establishment of four ES cell lines from the Syrian "golden" hamster (Mesocricetus auratus) is described. The cells can be maintained in the undifferentiated state when grown on primary mouse embryonic fibroblast feeder layers. In suspension culture they spontaneously differentiate into embryoid bodies of increasing complexity which contain a variety of tissues including embryonic ectoderm and myocardium. All four lines-one female and three male-are karyotypically normal with 44 chromosomes. Hamster is the second species from which ES cells have been established. As in mouse, the cells should be useful for developmental and transgenic studies.

Original languageEnglish (US)
Pages (from-to)224-227
Number of pages4
JournalDevelopmental biology
Volume127
Issue number1
DOIs
StatePublished - May 1988
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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