Establishment of two new cell lines derived from human breast carcinomas with her-2/neu amplification

P. Meltzer, A. Leibovitz, W. Dalton, H. Villar, T. Kute, J. Davis, R. Nagle, J. Trent

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Abstract

Two human cell lines (UACC-812 and 893), both containing significant amplification of the HER-2/neu gene, were established from biopsy specimens of breast carcinomas. One patient had Stage II breast carcinoma; the other had metastatic disease. Characterisation of these lines has revealed that both are highly aneuploid containing multiple clonal chromosome alterations, have doubling times near 100 h, and are oestrogen and progesterone receptor negative. Electron microscopy demonstrates that both lines contain numerous microvilli, cytoplasmic filaments, multivesicular bodies, and desmosomes. Immunoblot analysis for P-glycoprotein using the monoclonal antibody C219 was negative for both patient cell lines. These relatively rare cell lines may represent a useful model to investigate human breast carcinomas.

Original languageEnglish (US)
Pages (from-to)727-735
Number of pages9
JournalBritish journal of cancer
Volume63
Issue number5
DOIs
StatePublished - May 1991

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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