Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification

P. Meltzer, A. Leibovitz, W. Dalton, H. Villar, T. Kute, J. Davis, Raymond B Nagle, J. Trent

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Two human cell lines (UACC-812 and 893), both containing significant amplification of the HER-2/neu gene, were established from biopsy specimens of breast carcinomas. One patient had Stage II breast carcinoma; the other had metastatic disease. Characterisation of these lines has revealed that both are highly aneuploid containing multiple clonal chromosome alterations, have doubling times near 100 h, and are oestrogen and progesterone receptor negative. Electron microscopy demonstrates that both lines contain numerous microvilli, cytoplasmic filaments, multivesicular bodies, and desmosomes. Immunoblot analysis for P-glycoprotein using the monoclonal antibody C219 was negative for both patient cell lines. These relatively rare cell lines may represent a useful model to investigate human breast carcinomas.

Original languageEnglish (US)
Pages (from-to)727-735
Number of pages9
JournalBritish Journal of Cancer
Volume63
Issue number5
StatePublished - 1991
Externally publishedYes

Fingerprint

erbB-2 Genes
Breast Neoplasms
Cell Line
Multivesicular Bodies
Desmosomes
P-Glycoprotein
Aneuploidy
Progesterone Receptors
Microvilli
Cytoskeleton
Estrogen Receptors
Electron Microscopy
Chromosomes
Monoclonal Antibodies
Biopsy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Meltzer, P., Leibovitz, A., Dalton, W., Villar, H., Kute, T., Davis, J., ... Trent, J. (1991). Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. British Journal of Cancer, 63(5), 727-735.

Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. / Meltzer, P.; Leibovitz, A.; Dalton, W.; Villar, H.; Kute, T.; Davis, J.; Nagle, Raymond B; Trent, J.

In: British Journal of Cancer, Vol. 63, No. 5, 1991, p. 727-735.

Research output: Contribution to journalArticle

Meltzer, P, Leibovitz, A, Dalton, W, Villar, H, Kute, T, Davis, J, Nagle, RB & Trent, J 1991, 'Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification', British Journal of Cancer, vol. 63, no. 5, pp. 727-735.
Meltzer P, Leibovitz A, Dalton W, Villar H, Kute T, Davis J et al. Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. British Journal of Cancer. 1991;63(5):727-735.
Meltzer, P. ; Leibovitz, A. ; Dalton, W. ; Villar, H. ; Kute, T. ; Davis, J. ; Nagle, Raymond B ; Trent, J. / Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. In: British Journal of Cancer. 1991 ; Vol. 63, No. 5. pp. 727-735.
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