Estimation of sperm numbers in insects by fluorometry

A. K. Reichardt, D. E. Wheeler

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

To facilitate the study of mating biology in the desert leaf-cutter ant Acromyrmex versicolor, methods were developed that allowed storage and easy quantification of sperm samples collected from both male and female reproductive tracts. Sperm samples stored frozen were sonicated, stained with a fluorescent DNA stain, and the fluorescence emitted by the stained sperm heads was measured. The intensity of fluorescence was shown to be a linear function of the number of sperm in the sample as determined by counting.

Original languageEnglish (US)
Pages (from-to)449-452
Number of pages4
JournalInsectes Sociaux
Volume42
Issue number4
DOIs
StatePublished - Dec 1 1995

Keywords

  • Sperm quantification
  • ants
  • fluorometry
  • sperm counts

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

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