Estrogen and progesterone regulation of human fibroblast-like synoviocyte function in vitro: Implications in rheumatoid arthritis

Zhila Khalkhali-Ellis, Elisabeth A. Seftor, Daniel R C Nieva, Robert J Handa, Richard H. Price, Dawn A. Kirschmann, Vijaykumar M. Baragi, Ram V. Sharma, Ramesh C. Bhalla, Terry L. Moore, Mary J C Hendrix

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Objective. Despite increasing evidence regarding the significance of sex hormones in rheumatoid arthritis (RA), their etiopathological role and potential longterm effect on joint destruction remain unclear. We hypothesized that estrogen receptors (ER-α) are present in fibroblast-like synoviocytes, and 17β-estradiol can modulate the production and activity of matrix degrading enzymes produced by these cells. Thus, depending on the endocrine balance, fibroblast-like synoviocyte activity can be suppressed or enhanced, leading to amelioration or exacerbation of the disease process, respectively. Methods. By utilizing an in vitro cartilage invasion model, in combination with the molecular analyses of hormone receptors, matrix metalloproteinases (MMP) and their respective inhibitors, we investigated the effect of hormones (i.e., estrogen and progesterone) on fibroblast-like synoviocyte phenotypic changes, with particular emphasis on their functional interactions with cartilage. Results. Our studies reveal the presence of functional ER-α in fibroblast-like synoviocytes. The findings indicate that estrogen exerts a stimulatory effect, while progesterone has an inhibitory effect on the expression of MMP, their tissue inhibitors (TIMP), and enzymatic activity of MMP produced by these cells. Furthermore, transfection of fibroblast-like synoviocytes with the ER-α gene resulted in the increased degradation and invasion of cartilage. Conclusion. We identified the presence of functional ER-α in fibroblast-like synoviocytes. This renders fibroblast- like synoviocytes as target cells for hormonal regulation. The regulatory effect of estrogen is partly targeted to the MMP and their respective inhibitors associated with fibroblast-like synoviocytes. Such studies provide a link between hormonal status and disease activity in RA and open new venues for future therapeutic intervention to combat this debilitating disease.

Original languageEnglish (US)
Pages (from-to)1622-1631
Number of pages10
JournalJournal of Rheumatology
Volume27
Issue number7
StatePublished - 2000
Externally publishedYes

Fingerprint

Progesterone
Rheumatoid Arthritis
Estrogens
Fibroblasts
Matrix Metalloproteinases
Estrogen Receptors
Cartilage
Hormones
Synoviocytes
In Vitro Techniques
Gonadal Steroid Hormones
Transfection
Disease Progression
Estradiol
Joints
Enzymes
Genes

Keywords

  • Estrogen receptor-α
  • Matrix metalloproteinases
  • Rheumatoid arthritis
  • Sex hormones
  • Synoviocytes
  • Timp

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Khalkhali-Ellis, Z., Seftor, E. A., Nieva, D. R. C., Handa, R. J., Price, R. H., Kirschmann, D. A., ... Hendrix, M. J. C. (2000). Estrogen and progesterone regulation of human fibroblast-like synoviocyte function in vitro: Implications in rheumatoid arthritis. Journal of Rheumatology, 27(7), 1622-1631.

Estrogen and progesterone regulation of human fibroblast-like synoviocyte function in vitro : Implications in rheumatoid arthritis. / Khalkhali-Ellis, Zhila; Seftor, Elisabeth A.; Nieva, Daniel R C; Handa, Robert J; Price, Richard H.; Kirschmann, Dawn A.; Baragi, Vijaykumar M.; Sharma, Ram V.; Bhalla, Ramesh C.; Moore, Terry L.; Hendrix, Mary J C.

In: Journal of Rheumatology, Vol. 27, No. 7, 2000, p. 1622-1631.

Research output: Contribution to journalArticle

Khalkhali-Ellis, Z, Seftor, EA, Nieva, DRC, Handa, RJ, Price, RH, Kirschmann, DA, Baragi, VM, Sharma, RV, Bhalla, RC, Moore, TL & Hendrix, MJC 2000, 'Estrogen and progesterone regulation of human fibroblast-like synoviocyte function in vitro: Implications in rheumatoid arthritis', Journal of Rheumatology, vol. 27, no. 7, pp. 1622-1631.
Khalkhali-Ellis, Zhila ; Seftor, Elisabeth A. ; Nieva, Daniel R C ; Handa, Robert J ; Price, Richard H. ; Kirschmann, Dawn A. ; Baragi, Vijaykumar M. ; Sharma, Ram V. ; Bhalla, Ramesh C. ; Moore, Terry L. ; Hendrix, Mary J C. / Estrogen and progesterone regulation of human fibroblast-like synoviocyte function in vitro : Implications in rheumatoid arthritis. In: Journal of Rheumatology. 2000 ; Vol. 27, No. 7. pp. 1622-1631.
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abstract = "Objective. Despite increasing evidence regarding the significance of sex hormones in rheumatoid arthritis (RA), their etiopathological role and potential longterm effect on joint destruction remain unclear. We hypothesized that estrogen receptors (ER-α) are present in fibroblast-like synoviocytes, and 17β-estradiol can modulate the production and activity of matrix degrading enzymes produced by these cells. Thus, depending on the endocrine balance, fibroblast-like synoviocyte activity can be suppressed or enhanced, leading to amelioration or exacerbation of the disease process, respectively. Methods. By utilizing an in vitro cartilage invasion model, in combination with the molecular analyses of hormone receptors, matrix metalloproteinases (MMP) and their respective inhibitors, we investigated the effect of hormones (i.e., estrogen and progesterone) on fibroblast-like synoviocyte phenotypic changes, with particular emphasis on their functional interactions with cartilage. Results. Our studies reveal the presence of functional ER-α in fibroblast-like synoviocytes. The findings indicate that estrogen exerts a stimulatory effect, while progesterone has an inhibitory effect on the expression of MMP, their tissue inhibitors (TIMP), and enzymatic activity of MMP produced by these cells. Furthermore, transfection of fibroblast-like synoviocytes with the ER-α gene resulted in the increased degradation and invasion of cartilage. Conclusion. We identified the presence of functional ER-α in fibroblast-like synoviocytes. This renders fibroblast- like synoviocytes as target cells for hormonal regulation. The regulatory effect of estrogen is partly targeted to the MMP and their respective inhibitors associated with fibroblast-like synoviocytes. Such studies provide a link between hormonal status and disease activity in RA and open new venues for future therapeutic intervention to combat this debilitating disease.",
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T1 - Estrogen and progesterone regulation of human fibroblast-like synoviocyte function in vitro

T2 - Implications in rheumatoid arthritis

AU - Khalkhali-Ellis, Zhila

AU - Seftor, Elisabeth A.

AU - Nieva, Daniel R C

AU - Handa, Robert J

AU - Price, Richard H.

AU - Kirschmann, Dawn A.

AU - Baragi, Vijaykumar M.

AU - Sharma, Ram V.

AU - Bhalla, Ramesh C.

AU - Moore, Terry L.

AU - Hendrix, Mary J C

PY - 2000

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N2 - Objective. Despite increasing evidence regarding the significance of sex hormones in rheumatoid arthritis (RA), their etiopathological role and potential longterm effect on joint destruction remain unclear. We hypothesized that estrogen receptors (ER-α) are present in fibroblast-like synoviocytes, and 17β-estradiol can modulate the production and activity of matrix degrading enzymes produced by these cells. Thus, depending on the endocrine balance, fibroblast-like synoviocyte activity can be suppressed or enhanced, leading to amelioration or exacerbation of the disease process, respectively. Methods. By utilizing an in vitro cartilage invasion model, in combination with the molecular analyses of hormone receptors, matrix metalloproteinases (MMP) and their respective inhibitors, we investigated the effect of hormones (i.e., estrogen and progesterone) on fibroblast-like synoviocyte phenotypic changes, with particular emphasis on their functional interactions with cartilage. Results. Our studies reveal the presence of functional ER-α in fibroblast-like synoviocytes. The findings indicate that estrogen exerts a stimulatory effect, while progesterone has an inhibitory effect on the expression of MMP, their tissue inhibitors (TIMP), and enzymatic activity of MMP produced by these cells. Furthermore, transfection of fibroblast-like synoviocytes with the ER-α gene resulted in the increased degradation and invasion of cartilage. Conclusion. We identified the presence of functional ER-α in fibroblast-like synoviocytes. This renders fibroblast- like synoviocytes as target cells for hormonal regulation. The regulatory effect of estrogen is partly targeted to the MMP and their respective inhibitors associated with fibroblast-like synoviocytes. Such studies provide a link between hormonal status and disease activity in RA and open new venues for future therapeutic intervention to combat this debilitating disease.

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KW - Matrix metalloproteinases

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KW - Sex hormones

KW - Synoviocytes

KW - Timp

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