Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells

Barry S. Komm, Christopher M. Terpening, David J. Benz, Kimberlie A. Graeme, Alfred Gallegos, Murray Korc, Geoffrey L. Greene, Bert W. O'Malley, Mark R Haussler

Research output: Contribution to journalArticle

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Abstract

High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant ≅ 1.0 nM) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-β messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 nM estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalScience
Volume241
Issue number4861
StatePublished - 1988

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Osteosarcoma
Osteoblasts
Estrogen Receptors
Messenger RNA
Estradiol
Estrogens
Extracellular Matrix Proteins
DNA Probes
Transforming Growth Factors
Collagen Type I
Iodine
Testosterone
Complementary DNA
Steroids
Binding Sites
Maintenance
RNA

ASJC Scopus subject areas

  • General

Cite this

Komm, B. S., Terpening, C. M., Benz, D. J., Graeme, K. A., Gallegos, A., Korc, M., ... Haussler, M. R. (1988). Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. Science, 241(4861), 81-84.

Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. / Komm, Barry S.; Terpening, Christopher M.; Benz, David J.; Graeme, Kimberlie A.; Gallegos, Alfred; Korc, Murray; Greene, Geoffrey L.; O'Malley, Bert W.; Haussler, Mark R.

In: Science, Vol. 241, No. 4861, 1988, p. 81-84.

Research output: Contribution to journalArticle

Komm, BS, Terpening, CM, Benz, DJ, Graeme, KA, Gallegos, A, Korc, M, Greene, GL, O'Malley, BW & Haussler, MR 1988, 'Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells', Science, vol. 241, no. 4861, pp. 81-84.
Komm BS, Terpening CM, Benz DJ, Graeme KA, Gallegos A, Korc M et al. Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. Science. 1988;241(4861):81-84.
Komm, Barry S. ; Terpening, Christopher M. ; Benz, David J. ; Graeme, Kimberlie A. ; Gallegos, Alfred ; Korc, Murray ; Greene, Geoffrey L. ; O'Malley, Bert W. ; Haussler, Mark R. / Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. In: Science. 1988 ; Vol. 241, No. 4861. pp. 81-84.
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