Estrogen regulation of the brain renin-angiotensin system in protection against angiotensin II-induced sensitization of hypertension

Baojian Xue, Zhongming Zhang, Terry G. Beltz, Fang Guo, Meredith Hay, Alan Kim Johnson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

This study investigated sex differences in the sensitization of angiotensin (ANG) II-induced hypertension and the role of central estrogen and ANG- (1-7) in this process. Male and female rats were implanted for telemetered blood pressure (BP) recording. A subcutaneous subpressor dose of ANG II was given alone or concurrently with intracerebroventricular estrogen, ANG-(1-7), an ANG-(1-7) receptor antagonist A-779 or vehicle for 1 wk (induction). After a 1-wk rest (delay), a pressor dose of ANG II was given for 2 wk (expression). In males and ovariectomized females, subpressor ANG II had no sustained effect on BP during induction, but produced an enhanced hypertensive response to the subsequent pressor dose of ANG II during expression. Central administration of estrogen or ANG-(1-7) during induction blocked ANG II-induced sensitization. In intact females, subpressor ANG II treatment produced a decrease in BP during induction and delay, and subsequent pressor ANG II treatment given during expression produced only a slight but significant increase in BP. However, central blockade of ANG-(1-7) by intracerebroventricular infusion of A-779 during induction restored the decreased BP observed in females during induction and enhanced the pressor response to the ANG II treatment during expression. RT-PCR analyses indicated that estrogen given during induction upregulated mRNA expression of the renin-angiotensin system (RAS) antihypertensive components, whereas both central estrogen and ANG-(1-7) downregulated mRNA expression of RAS hypertensive components in the lamina terminalis. The results indicate that females are protected from ANG II-induced sensitization through central estrogen and its regulation of brain RAS.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume307
Issue number2
DOIs
StatePublished - Jul 15 2014

Fingerprint

Renin-Angiotensin System
Angiotensin II
Estrogens
Hypertension
Brain
7-Ala-angiotensin (1-7)
Blood Pressure
Central Nervous System Sensitization
Intraventricular Infusions
Messenger RNA
Sex Characteristics
Antihypertensive Agents
Hypothalamus
angiotensin I (1-7)
Therapeutics
Down-Regulation
Polymerase Chain Reaction

Keywords

  • Angiotensin II
  • Angiotensin-(1-7)
  • Blood pressure
  • Central nervous system
  • Sex difference

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Estrogen regulation of the brain renin-angiotensin system in protection against angiotensin II-induced sensitization of hypertension. / Xue, Baojian; Zhang, Zhongming; Beltz, Terry G.; Guo, Fang; Hay, Meredith; Johnson, Alan Kim.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 307, No. 2, 15.07.2014.

Research output: Contribution to journalArticle

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