Ethanol fixation of bladder irrigation specimens for flow cytometric analysis. A multiinstitutional study from the Bladder Cancer Flow Cytometry Network

D. K. Hermansen, M. R. Melamed, J. S. Coon, Ronald S Weinstein, R. DeVere White, A. D. Deitch, L. L. Wheeless, J. E. Reeder, R. Wersto, L. G. Koss

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ethanol preservation of voided and catheterized urine has long been the standard for urinary cytologic study. In this report ethanol preservation of bladder irrigation specimens was evaluated for flow cytometric analysis in a multiinstitutional study requiring specimen transport. Specimens from ten patients obtained at one center were preserved for varying periods of time in 50% ethanol, then distributed by express mail to four other participating centers, up to 3000 miles distant. On receipt, from 1 to 3 weeks after collection, the samples were processed and examined by flow cytometric study using propidium iodide as the fluorochrome. Forty-six of the 50 (92%) analyzed specimens gave satisfactory histograms. In 41 of the 46 adequate samples (89%), an aneuploid peak in the alcohol preserved (propidium iodide stained) specimen correlated well with the fresh (acridine orange stained) specimen. However, there was variable loss of DNA stainability with a broadening of the coefficient of variation in some alcohol-preserved specimens and an increase in cellular debris so that measurements of DNA index and percent hyperdiploid cells were considered unreliable. The authors conclude that ethanol preservation of bladder irrigation specimens for short periods of time may be a feasible alternative when flow cytometric analysis cannot be carried out on fresh specimens, but that this is not optimal fixation for specimens that must be transported and further studies of other fixatives are recommended.

Original languageEnglish (US)
Pages (from-to)1780-1783
Number of pages4
JournalCancer
Volume63
Issue number9
StatePublished - 1989
Externally publishedYes

Fingerprint

Urinary Bladder Neoplasms
Flow Cytometry
Urinary Bladder
Ethanol
Propidium
Alcohols
Fixatives
Acridine Orange
Polyploidy
DNA
Postal Service
Aneuploidy
Fluorescent Dyes
Urine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hermansen, D. K., Melamed, M. R., Coon, J. S., Weinstein, R. S., DeVere White, R., Deitch, A. D., ... Koss, L. G. (1989). Ethanol fixation of bladder irrigation specimens for flow cytometric analysis. A multiinstitutional study from the Bladder Cancer Flow Cytometry Network. Cancer, 63(9), 1780-1783.

Ethanol fixation of bladder irrigation specimens for flow cytometric analysis. A multiinstitutional study from the Bladder Cancer Flow Cytometry Network. / Hermansen, D. K.; Melamed, M. R.; Coon, J. S.; Weinstein, Ronald S; DeVere White, R.; Deitch, A. D.; Wheeless, L. L.; Reeder, J. E.; Wersto, R.; Koss, L. G.

In: Cancer, Vol. 63, No. 9, 1989, p. 1780-1783.

Research output: Contribution to journalArticle

Hermansen, DK, Melamed, MR, Coon, JS, Weinstein, RS, DeVere White, R, Deitch, AD, Wheeless, LL, Reeder, JE, Wersto, R & Koss, LG 1989, 'Ethanol fixation of bladder irrigation specimens for flow cytometric analysis. A multiinstitutional study from the Bladder Cancer Flow Cytometry Network', Cancer, vol. 63, no. 9, pp. 1780-1783.
Hermansen, D. K. ; Melamed, M. R. ; Coon, J. S. ; Weinstein, Ronald S ; DeVere White, R. ; Deitch, A. D. ; Wheeless, L. L. ; Reeder, J. E. ; Wersto, R. ; Koss, L. G. / Ethanol fixation of bladder irrigation specimens for flow cytometric analysis. A multiinstitutional study from the Bladder Cancer Flow Cytometry Network. In: Cancer. 1989 ; Vol. 63, No. 9. pp. 1780-1783.
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