Ethanol-induced alterations in human erythrocyte shape and surface properties: Modulatory role of prostaglandin E1

Ronald W. McLawhon, Yehuda Marikovsky, Neil J. Thomas, Ronald S. Weinstein

Research output: Contribution to journalArticle

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Abstract

Exposure of human erythrocytes to ethanol (1 to 20% by vol) in Ca2+ and Mg2+-free phosphate-buffered saline, pH 7.4, transformed biconcave discs into spiculated echinocytes within 3 min at 25°C. The effects of ethanol were concentration- and time-dependent, but reversible by washing in the incubation buffer system within 60 min of initial exposure to ethanol. After prolonged ethanol exposure (180 min), washing of cells resulted in the formation of stomatocytes (cup-forms). Ethanol-induced echinocytosis was also accompanied by a 30% enhancement in the agglutinability of erythrocytes by ligands with high affinity for negative surface charge (poly-l-lysine and wheat germ agglutinin, 20 μl/ml) without any alterations in surface charge topography. Concomitant exposure of erythrocytes to prostaglandin E1 (100 nm) selectively prevented the enhancement of ligand-mediated agglutinability, but did not modify cell shape. These data indicate that certain erythrocyte surface properties may not be directly influenced by cell shape and suggest a unique modulatory action of prostaglandin E1 on shape-transformed cells.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalThe Journal of Membrane Biology
Volume99
Issue number1
DOIs
StatePublished - Feb 1 1987

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Keywords

  • cell shape control
  • ligand-mediated agglutinability
  • lipid bilayer
  • membrane skeleton
  • surface charge topography

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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