ETS family protein ETV2 is required for initiation of the endothelial lineage but not the hematopoietic lineage in the Xenopus embryo

Matthew C. Salanga, Stryder M. Meadows, Candace T. Myers, Paul A Krieg

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Transcription factors of the ETS family are important regulators of endothelial and hematopoietic development. We have characterized the Xenopus orthologue of the ETS transcription factor, ETV2. Expression analysis shows that etv2 is highly expressed in hematopoietic and endothelial precursor cells in the Xenopus embryo. In gain-of-function experiments, ETV2 is sufficient to activate ectopic expression of vascular endothelial markers. In addition, ETV2 activated expression of hematopoietic genes representing the myeloid but not the erythroid lineage. Loss-of-function studies indicate that ETV2 is required for expression of all endothelial markers examined. However, knockdown of ETV2 has no detectable effects on expression of either myeloid or erythroid markers. This contrasts with studies in mouse and zebrafish where ETV2 is required for development of the myeloid lineage. Our studies confirm an essential role for ETV2 in endothelial development, but also reveal important differences in hematopoietic development between organisms.

Original languageEnglish (US)
Pages (from-to)1178-1187
Number of pages10
JournalDevelopmental Dynamics
Volume239
Issue number4
DOIs
StatePublished - Apr 2010

Fingerprint

Xenopus
Transcription Factors
Embryonic Structures
Zebrafish
Blood Vessels
Proteins
Endothelial Cells
Gene Expression
Ectopic Gene Expression

Keywords

  • Dexamethasone-inducible
  • ER71
  • Erythroid
  • Etsrp
  • Morpholino
  • Myeloid

ASJC Scopus subject areas

  • Developmental Biology

Cite this

ETS family protein ETV2 is required for initiation of the endothelial lineage but not the hematopoietic lineage in the Xenopus embryo. / Salanga, Matthew C.; Meadows, Stryder M.; Myers, Candace T.; Krieg, Paul A.

In: Developmental Dynamics, Vol. 239, No. 4, 04.2010, p. 1178-1187.

Research output: Contribution to journalArticle

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