Euglena gracilis chloroplast psbB, psbT, psbH and psbN gene cluster: Regulation of psbB-psbT pre-mRNA processing

Ling Hong, Jennifer K. Stevenson, William B Roth, Richard B. Hallick

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A 2.4 kb region of the Euglena gracilis chloroplast genome containing the genes psbT, psbH and psbN was characterized. The mRNAs transcribed from psbB, psbT, psbH and psbN were analyzed by northern hybridization, S1 nuclease protection analysis and primer extension RNA sequencing. The gene pairs psbB psbT and psbH-psbN are cotranscribed from opposite strands. The 5′ end of the psbN-psbH transcript and the intercistronic cleavage sites between psbB-psbT and psbN-psbH were determined. The extent of psbB-psbT intercistronic cleavage is greater during photoautotrophic than heterotrophic growth and thus may be developmentally regulated. Processing is absent in the nonphotosynthetic E. gracilis mutant Y9Z1NaL.

Original languageEnglish (US)
Pages (from-to)180-188
Number of pages9
JournalMGG Molecular & General Genetics
Volume247
Issue number2
DOIs
StatePublished - Mar 1995

Fingerprint

Euglena gracilis
RNA Precursors
Chloroplasts
Multigene Family
Heterotrophic Processes
Chloroplast Genome
RNA Sequence Analysis
Genes
Messenger RNA

Keywords

  • Euglena gracilis chloroplast
  • Evolution of psbB operon
  • Light induction
  • Photosystem II polypeptides
  • RNA processing

ASJC Scopus subject areas

  • Genetics

Cite this

Euglena gracilis chloroplast psbB, psbT, psbH and psbN gene cluster : Regulation of psbB-psbT pre-mRNA processing. / Hong, Ling; Stevenson, Jennifer K.; Roth, William B; Hallick, Richard B.

In: MGG Molecular & General Genetics, Vol. 247, No. 2, 03.1995, p. 180-188.

Research output: Contribution to journalArticle

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AU - Roth, William B

AU - Hallick, Richard B.

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N2 - A 2.4 kb region of the Euglena gracilis chloroplast genome containing the genes psbT, psbH and psbN was characterized. The mRNAs transcribed from psbB, psbT, psbH and psbN were analyzed by northern hybridization, S1 nuclease protection analysis and primer extension RNA sequencing. The gene pairs psbB psbT and psbH-psbN are cotranscribed from opposite strands. The 5′ end of the psbN-psbH transcript and the intercistronic cleavage sites between psbB-psbT and psbN-psbH were determined. The extent of psbB-psbT intercistronic cleavage is greater during photoautotrophic than heterotrophic growth and thus may be developmentally regulated. Processing is absent in the nonphotosynthetic E. gracilis mutant Y9Z1NaL.

AB - A 2.4 kb region of the Euglena gracilis chloroplast genome containing the genes psbT, psbH and psbN was characterized. The mRNAs transcribed from psbB, psbT, psbH and psbN were analyzed by northern hybridization, S1 nuclease protection analysis and primer extension RNA sequencing. The gene pairs psbB psbT and psbH-psbN are cotranscribed from opposite strands. The 5′ end of the psbN-psbH transcript and the intercistronic cleavage sites between psbB-psbT and psbN-psbH were determined. The extent of psbB-psbT intercistronic cleavage is greater during photoautotrophic than heterotrophic growth and thus may be developmentally regulated. Processing is absent in the nonphotosynthetic E. gracilis mutant Y9Z1NaL.

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