Evaluating the protective role of ischaemic preconditioning in rat hearts using a stationary small-animal SPECT imager and 99mTc-glucarate

Zhonglin Liu, Harrison H Barrett, Gail D. Stevenson, Lars R Furenlid, Koon Yan Pak, James M. Woolfenden

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

OBJECTIVE: To examine the protective role of ischaemic preconditioning (IPC) in rat hearts using Tc-glucarate (GLA) and a stationary SPECT imager, FastSPECT. METHODS: Twenty-four rats with 30 min myocardial ischaemia and 150 min reperfusion (IR) were studied as follows. The IPC group (n=6) underwent IPC (five cycles of 4 min ligation of the left coronary artery and reflow) before IR. The control group (n=7) was treated by IR without IPC. The SPT group (n=6) was subjected to IPC and an adenosine antagonist, 8-(p-sulfophenyl)-theophylline (SPT). The vehicle group (n=5) received IPC and SPT carrier vehicle. GLA was delivered intravenously 30 min post-reperfusion, and 2-h dynamic cardiac images were acquired by FastSPECT. RESULTS: GLA showed 'hot-spot' accumulation in the ischaemic area-at-risk (IAR) and exhibited lower retention (% 5 min peak) in the IPC and vehicle groups (33.8±2.6 vs. 35.7±9.2, P>0.05) than in the control and SPT groups (63.1±5.3 vs. 54.8±4.8, P>0.05). The infarct size (% IAR) was larger in the control and SPT groups (48.2±6.3 vs. 41.7±6.3, P>0.05) than that in the IPC and vehicle groups (21.0±1.9 vs. 19.1±4.6, P>0.05). In terms of the ex-vivo IAR-to-normal radioactivity ratio, there was a statistical difference between the control and IPC groups (7.4±0.9 vs. 3.0±0.4), as well as the SPT and vehicle groups (7.4±1.0 vs. 3.4±0.5). CONCLUSION: IPC offers cardioprotection and relates to the activation of adenosine receptors in rat hearts. FastSPECT GLA imaging is not only useful in detecting early ischaemia-reperfusion injury, but also valuable in evaluating cardioprotection.

Original languageEnglish (US)
Pages (from-to)120-128
Number of pages9
JournalNuclear Medicine Communications
Volume29
Issue number2
DOIs
StatePublished - Feb 2008

Fingerprint

Ischemic Preconditioning
Single-Photon Emission-Computed Tomography
Theophylline
Reperfusion
technetium Tc 99m glucarate
Purinergic P1 Receptors
Reperfusion Injury
Adenosine
Radioactivity
Myocardial Ischemia
Ligation
Coronary Vessels

Keywords

  • Tc- glucarate
  • Heart
  • Ischaemic preconditioning
  • Rat
  • Small-animal SPECT

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Evaluating the protective role of ischaemic preconditioning in rat hearts using a stationary small-animal SPECT imager and 99mTc-glucarate. / Liu, Zhonglin; Barrett, Harrison H; Stevenson, Gail D.; Furenlid, Lars R; Pak, Koon Yan; Woolfenden, James M.

In: Nuclear Medicine Communications, Vol. 29, No. 2, 02.2008, p. 120-128.

Research output: Contribution to journalArticle

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abstract = "OBJECTIVE: To examine the protective role of ischaemic preconditioning (IPC) in rat hearts using Tc-glucarate (GLA) and a stationary SPECT imager, FastSPECT. METHODS: Twenty-four rats with 30 min myocardial ischaemia and 150 min reperfusion (IR) were studied as follows. The IPC group (n=6) underwent IPC (five cycles of 4 min ligation of the left coronary artery and reflow) before IR. The control group (n=7) was treated by IR without IPC. The SPT group (n=6) was subjected to IPC and an adenosine antagonist, 8-(p-sulfophenyl)-theophylline (SPT). The vehicle group (n=5) received IPC and SPT carrier vehicle. GLA was delivered intravenously 30 min post-reperfusion, and 2-h dynamic cardiac images were acquired by FastSPECT. RESULTS: GLA showed 'hot-spot' accumulation in the ischaemic area-at-risk (IAR) and exhibited lower retention ({\%} 5 min peak) in the IPC and vehicle groups (33.8±2.6 vs. 35.7±9.2, P>0.05) than in the control and SPT groups (63.1±5.3 vs. 54.8±4.8, P>0.05). The infarct size ({\%} IAR) was larger in the control and SPT groups (48.2±6.3 vs. 41.7±6.3, P>0.05) than that in the IPC and vehicle groups (21.0±1.9 vs. 19.1±4.6, P>0.05). In terms of the ex-vivo IAR-to-normal radioactivity ratio, there was a statistical difference between the control and IPC groups (7.4±0.9 vs. 3.0±0.4), as well as the SPT and vehicle groups (7.4±1.0 vs. 3.4±0.5). CONCLUSION: IPC offers cardioprotection and relates to the activation of adenosine receptors in rat hearts. FastSPECT GLA imaging is not only useful in detecting early ischaemia-reperfusion injury, but also valuable in evaluating cardioprotection.",
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AU - Pak, Koon Yan

AU - Woolfenden, James M.

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N2 - OBJECTIVE: To examine the protective role of ischaemic preconditioning (IPC) in rat hearts using Tc-glucarate (GLA) and a stationary SPECT imager, FastSPECT. METHODS: Twenty-four rats with 30 min myocardial ischaemia and 150 min reperfusion (IR) were studied as follows. The IPC group (n=6) underwent IPC (five cycles of 4 min ligation of the left coronary artery and reflow) before IR. The control group (n=7) was treated by IR without IPC. The SPT group (n=6) was subjected to IPC and an adenosine antagonist, 8-(p-sulfophenyl)-theophylline (SPT). The vehicle group (n=5) received IPC and SPT carrier vehicle. GLA was delivered intravenously 30 min post-reperfusion, and 2-h dynamic cardiac images were acquired by FastSPECT. RESULTS: GLA showed 'hot-spot' accumulation in the ischaemic area-at-risk (IAR) and exhibited lower retention (% 5 min peak) in the IPC and vehicle groups (33.8±2.6 vs. 35.7±9.2, P>0.05) than in the control and SPT groups (63.1±5.3 vs. 54.8±4.8, P>0.05). The infarct size (% IAR) was larger in the control and SPT groups (48.2±6.3 vs. 41.7±6.3, P>0.05) than that in the IPC and vehicle groups (21.0±1.9 vs. 19.1±4.6, P>0.05). In terms of the ex-vivo IAR-to-normal radioactivity ratio, there was a statistical difference between the control and IPC groups (7.4±0.9 vs. 3.0±0.4), as well as the SPT and vehicle groups (7.4±1.0 vs. 3.4±0.5). CONCLUSION: IPC offers cardioprotection and relates to the activation of adenosine receptors in rat hearts. FastSPECT GLA imaging is not only useful in detecting early ischaemia-reperfusion injury, but also valuable in evaluating cardioprotection.

AB - OBJECTIVE: To examine the protective role of ischaemic preconditioning (IPC) in rat hearts using Tc-glucarate (GLA) and a stationary SPECT imager, FastSPECT. METHODS: Twenty-four rats with 30 min myocardial ischaemia and 150 min reperfusion (IR) were studied as follows. The IPC group (n=6) underwent IPC (five cycles of 4 min ligation of the left coronary artery and reflow) before IR. The control group (n=7) was treated by IR without IPC. The SPT group (n=6) was subjected to IPC and an adenosine antagonist, 8-(p-sulfophenyl)-theophylline (SPT). The vehicle group (n=5) received IPC and SPT carrier vehicle. GLA was delivered intravenously 30 min post-reperfusion, and 2-h dynamic cardiac images were acquired by FastSPECT. RESULTS: GLA showed 'hot-spot' accumulation in the ischaemic area-at-risk (IAR) and exhibited lower retention (% 5 min peak) in the IPC and vehicle groups (33.8±2.6 vs. 35.7±9.2, P>0.05) than in the control and SPT groups (63.1±5.3 vs. 54.8±4.8, P>0.05). The infarct size (% IAR) was larger in the control and SPT groups (48.2±6.3 vs. 41.7±6.3, P>0.05) than that in the IPC and vehicle groups (21.0±1.9 vs. 19.1±4.6, P>0.05). In terms of the ex-vivo IAR-to-normal radioactivity ratio, there was a statistical difference between the control and IPC groups (7.4±0.9 vs. 3.0±0.4), as well as the SPT and vehicle groups (7.4±1.0 vs. 3.4±0.5). CONCLUSION: IPC offers cardioprotection and relates to the activation of adenosine receptors in rat hearts. FastSPECT GLA imaging is not only useful in detecting early ischaemia-reperfusion injury, but also valuable in evaluating cardioprotection.

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