Evaluation of a prototype real-time PCR assay for carcinogenic human papillomavirus (HPV) detection and simultaneous HPV genotype 16 (HPV16) and HPV18 genotyping

Philip E. Castle, Mark Sadorra, Tiffany Lau, Carrie Aldrich, Francisco A Garcia, Janet Kornegay

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Results from a prototype real-time PCR assay that separately detected human papillomavirus genotype 16 (HPV16), HPV18, and 12 other carcinogenic HPV genotypes in aggregate (cobas 4800 HPV test) and results from a PCR assay that detects 37 HPV genotypes individually (Linear Array) were compared using a convenience sample of cervical specimens (n = 531). The percentage of total agreement between the two assays was 94.7% (95% confidence interval, 92.5 to 96.5%). The Linear Array test was more likely than cobas 4800 HPV test to test positive for the 12 other carcinogenic HPV genotypes among women without evidence of cervical disease (P = 0.004).

Original languageEnglish (US)
Pages (from-to)3344-3347
Number of pages4
JournalJournal of Clinical Microbiology
Volume47
Issue number10
DOIs
StatePublished - Oct 2009

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Human papillomavirus 16
Real-Time Polymerase Chain Reaction
Genotype
Confidence Intervals
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Evaluation of a prototype real-time PCR assay for carcinogenic human papillomavirus (HPV) detection and simultaneous HPV genotype 16 (HPV16) and HPV18 genotyping. / Castle, Philip E.; Sadorra, Mark; Lau, Tiffany; Aldrich, Carrie; Garcia, Francisco A; Kornegay, Janet.

In: Journal of Clinical Microbiology, Vol. 47, No. 10, 10.2009, p. 3344-3347.

Research output: Contribution to journalArticle

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